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mTB3-AviD tag

Plasmid

#210203

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

AviD-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pE5771

Plasmid

#170778

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

Kan resistance (kanR )

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pE5773

Plasmid

#170780

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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hp-66%GC-40bp

Plasmid

#169200

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-20bp

Plasmid

#169201

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-40bp

Plasmid

#169202

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-100%GC-20bp

Plasmid

#169203

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

100%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-Left-half-120bp

Plasmid

#169204

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Left-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-Right-half-120bp

Plasmid

#169205

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Right-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50-0-50%GC-Left-half-120bp

Plasmid

#169206

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Left-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50-0-50%GC-Right-half-120bp

Plasmid

#169207

Purpose

50-0-50%GC-Right-half-120bp-hairpin

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Right-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-20bp

Plasmid

#169196

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-40bp

Plasmid

#169197

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-40bp-Revbindsite

Plasmid

#169198

Purpose

Force-activating hairpin for 5'-to-3' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-40bp-hairpin-binding-site-reversed

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-66%GC-20bp

Plasmid

#169199

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-No5'ss-NoBS-NoPseudoBS

Plasmid

#100987

Purpose

RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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