We narrowed to 10,295 results for: otos

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pThom77.3

Plasmid

#101636

Purpose

Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Tags

6XHis

Expression

Bacterial

Mutation

K68M

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TAC-WTSI

Plasmid

#80486

Purpose

piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

WTSI

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAd388-BPTF-sh28

Plasmid

#83276

Purpose

shRNA targeting BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA to BPTF

Use

Adenoviral

Promoter

pUC ori

Available Since

Nov. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pES007 (pTet-qacR 5/pQacA-deGFP)

Plasmid

#69036

Purpose

reporter with qacR 5 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pQacA and pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pES003 (pTet-qacR 2)

Plasmid

#69032

Purpose

qacR 2 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoMyc'-cYAP-1

Plasmid

#66854

Purpose

Express Myc-tagged C.elegans YAP-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoMyc'-EGL-44

Plasmid

#66855

Purpose

Express Myc-tagged C.elegans EGL-44 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

EGL-44 (CELE_F28B12.2 Nematode)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
mCerulean-Zyxin-6

Plasmid

#55398

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Zyxin (ZYX Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
XE156 delta LZ-XDpr-CS2+

Plasmid

#16766

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

delta LZ-XDpr (dact1-b Frog)

Use

Xenopus expression

Mutation

deletion of first 129 amino acids, including the …

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE155 XDpr-delta PDZB-CS2+

Plasmid

#16767

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Mutation

last four amino acids deleted

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE154 XDpr-pt mutant-CS2+

Plasmid

#16768

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-pt mutant (dact1-b Frog)

Use

Xenopus expression

Mutation

last four amino acids mutated to MNTV instead of …

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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7xSMAD_RE::FLuc

Plasmid

#124531

Purpose

SMAD2 FLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

SMAD FLuc reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin β5-GFP

Plasmid

#205090

Purpose

Express GFP-tagged human integrin β5 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 5 (ITGB5 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CREB

Plasmid

#68550

Purpose

AAV expression of CREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

CREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-mCREB

Plasmid

#68551

Purpose

AAV expression of mCREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

mCREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

S133A

Promoter

CMV

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_g5-HT3.0

Plasmid

#208711

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE NFkB-TA-LUC-UBC-GFP-W

Plasmid

#49343

Purpose

pHAGE NFkB-TA-LUC-UBC-GFP-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

eGFP (eGFP )
Luciferase
NFkB consensus binding sequence
TA
UBC

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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