We narrowed to 18,992 results for: Met;
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Plasmid#174364PurposeExpression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Tags8xHis, MBP, and TEVExpressionBacterialPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E18: attB4-polyhedrinp>-attB5
Plasmid#162977PurposeGateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoterDepositorInsertAcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E21: attB4-FerHp>-attB5
Plasmid#162978PurposeGateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyAvailable SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-KRAB-MCS_N-term
Plasmid#136888PurposeDoxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertKRAB repressor domain
UseLentiviralTagsFlagExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MCS-KRAB_C-term
Plasmid#136889PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codonDepositorInsertKRAB repressor domain
UseLentiviralTagsNoneExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Nectin-2
Plasmid#170295PurposeA knock-out vector for the mouse Nectin-2DepositorInsertA gRNA targeting the mouse Nectin-2 gene.
UseCRISPR and LentiviralAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Necl-5
Plasmid#170294PurposeA knock-out vector for the mouse Necl-5DepositorInsertA gRNA targeting the mouse Necl-5 gene.
UseCRISPR and LentiviralAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC8
Plasmid#170757PurposeCo-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.DepositorTypeEmpty backboneTagssite 1- V5 tag and site 2- FLAG tagExpressionMammalianPromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_268-459_D
Plasmid#146119PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
ExpressionInsectAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r
Plasmid#162936PurposeGateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoterDepositorInsertTriple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E01: attB5r-meGFP-nostop-attB1r
Plasmid#162940PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Plasmid#162937PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E11: attB5r-RU5u-His6-nostop-attB1r
Plasmid#162953PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E25: attB4-mPol2p>-attB5
Plasmid#162981PurposeGateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Plasmid#162956PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHis6-Halotag fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Plasmid#162935PurposeGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoterDepositorInsertMaltose-binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E09: attB4-Grp78p>-attB5
Plasmid#162974PurposeGateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E09: attB5r-RU5u-FLAG-nostop-attB1r
Plasmid#162951PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertFLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E59: attB5r-RU5u-2xHA-nostop-attB1r
Plasmid#162966PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteinsDepositorInsertDual HA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Plasmid#162961PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteinsDepositorInsertStrep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E26: attB5r-RU5u-myc-nostop-attB1r
Plasmid#162960PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteinsDepositorInsertMyc epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E10: attB5r-RU5u-HA-nostop-attB1r
Plasmid#162952PurposeGateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteinsDepositorInsertHA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E11: attB4-Grp78p>-attB1r
Plasmid#162970PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E10: attB4-FerHp>-attB1r
Plasmid#162969PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E24: attB5r-RU5u-V5-nostop-attB1r
Plasmid#162959PurposeGateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteinsDepositorInsertV5 epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E34: attB5r-iRFP670-nostop-attB1r
Plasmid#162947PurposeGateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E01: attB4-polyhedrinp>His6-nostop-attB1r
Plasmid#162932PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion with AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C415-E01: attB4-stuffer-attB1r
Plasmid#162931PurposeGateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectorsDepositorInsertstuffer fragment for vector controls
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138742PurposeIntegration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertZF43x6-Compact
UseSynthetic BiologyExpressionMammalianAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAID5WY
Plasmid#165050PurposeIntegrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)
Plasmid#139237PurposemMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)
Plasmid#139234PurposemMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)
Plasmid#139233PurposemMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)
Plasmid#139232PurposemMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only