We narrowed to 17,258 results for: LEA
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Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPGK-empty
Plasmid#35094DepositorPublicationInsertdeletion of zinc fingers and nuclease
TagsHAExpressionMammalianMutationuse as a transfection balance for pPGK-ZFN plasmi…PromoterPGKAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term
Plasmid#182925PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneTagsAPEX2 and FLAGExpressionMammalianPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1/V5-His-TN-DROSHA
Plasmid#106252PurposeExpress TN-DROSHA in mammalian cellsDepositorInsertDrosha Ribonuclease III (DROSHA Human)
TagsV5-HisExpressionMammalianMutationE1045Q and E1222Q mutationsPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR45Q
Plasmid#92248PurposeHTT donor DNA with 45Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorMutationBarcode of wobble bases to prevent TALEN cleaving…Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE4999
Plasmid#107555PurposeExpresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.DepositorInserthFnCpf1(dead1)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationD917APromoterCMVAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN256
Plasmid#188472PurposescDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFPDepositorInserteGFP-WPRE-DAISY-4T
Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCNPpo6
Plasmid#46086DepositorInsertPpoI
TagsNLSExpressionMammalianPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCNCre3
Plasmid#46088DepositorInsertCreI
TagsNLSExpressionMammalianPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDY0312 PhiBT Integrase
Plasmid#179111PurposePhiBT Integrase expression vectorDepositorInsertPhiBT Integrase
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS1891
Plasmid#1363DepositorPublicationInsertNLS-EYFP
ExpressionYeastAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1
Plasmid#122084PurposeExpress M.EcoGII fused to TRF1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-TERF1
UseRetroviralTagsV5 tag on Nter of TERF1ExpressionBacterial and MammalianAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…MutationRemoved stop codon to fuse it to the 2A-eGFPnls i…Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRES proHB-EGF NC
Plasmid#11604DepositorInsertHB-EGF (HBEGF Human)
ExpressionMammalianMutationNon-cleavable HB-EGF: Pro149 to Thr160 replaced w…Available sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
Flag-Rap1b V12
Plasmid#118323PurposeExpression of bovine Rap1bDepositorAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDZ514 pURA MET25p PP7-PS-2x-yeGFP
Plasmid#72230PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
ExpressionYeastPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-N-Strep2
Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only