We narrowed to 15,822 results for: LEA

1. R26-H2B-EGFP HR donor vector

   Plasmid

   #137925

   Purpose

   R0SA26 knock-in vector with homologous recombination-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-H2B-EGFP-bpA with homology arms
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pClneoMyc human NDR1

   Plasmid

   #37023

   Depositor

   Yutaka Hata

   Article

   Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

   Insert

   NDR1 (STK38 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-p3E-HyPer7

   Plasmid

   #194289

   Purpose

   Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52546

   Purpose

   Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   PhiC31o integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-human-pro-IL-18

   Plasmid

   #214317

   Purpose

   Expression of gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC1-GluR1Ctail

   Plasmid

   #32441

   Depositor

   Robert Malinow

   Article

   Rumpel et al Science. 2005 Apr 1;308(5718):83-8. Epub

   Insert

   GluR1 (GRIA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains the last C-terminal 81 amino acids of th…

   Promoter

   CMV

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-TIGHT-EGFP-backward donor

   Plasmid

   #22077

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15-His-∆NGlpG

    Plasmid

    #155131

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-ChRger2-TS-EYFP

    Plasmid

    #127238

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZS*11_4enh

    Plasmid

    #149423

    Purpose

    Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Inserts

    rbcS-E9 enhancer
    35S core enhancer
    Cab-1 enhancer
    AB80 enhancer
    

    Use

    Tags

    T120>A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ND4.2-Left TALE-G1397-N-DddA11

    Plasmid

    #179681

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid

    #166504

    Purpose

    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

    Depositor

    Angelos Constantinou

    Article

    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

    Insert

    None
    

    Use

    Tags

    TurboID and mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. DYSF-Venus

    Plasmid

    #29768

    Depositor

    Steven Vogel

    Article

    Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

    Insert

    DYSF-Venus (DYSF Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-CD81-(TEV)-Cre

    Plasmid

    #167939

    Purpose

    human CD81-TEV cleavage site-Cre

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Use

    Tags

    Cre recombinase and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Use

    Tags

    3x-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-1_VNp6-DARPinOFF7

    Plasmid

    #182398

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 6 (VNp6)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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