Skip to main content
Addgene
Showing: 4921 - 4940 of 22169 results

  1. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid
    #82032
    Purpose
    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82032-plasmid-map-sequence-id-155382

    Enlarge

  2. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80065
    Purpose
    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Zic2a enhancer driving c-fos promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80065-plasmid-map-sequence-id-152617

    Enlarge

  3. pAF161

    Plasmid
    #135926
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCerulean3
    Use
    Transposon
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135926-plasmid-map-sequence-id-270079

    Enlarge

  4. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

    Enlarge

  7. pEcNucC

    Plasmid
    #214050
    Purpose
    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    EcNucC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    lacUV5 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214050-plasmid-map-sequence-id-428997

    Enlarge

  8. B-HypaR-SpCas9

    Plasmid
    #126764
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A; N692A; M694A; Q695A; H698A; amino acids 10…
    Promoter
    CBh
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126764-plasmid-map-sequence-id-410886

    Enlarge

  9. pGBT9-FHIP

    Plasmid
    #198527
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    FHIP (FHIP1B Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent mutations in codons 353 and 651 as well as…
    Promoter
    ADH1
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198527-plasmid-map-sequence-id-393106

    Enlarge

  10. pET24TEV-tSHIP1

    Plasmid
    #183770
    Purpose
    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP1 (INPP5D Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa1 to aa883 only
    Promoter
    T7 lac
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183770-plasmid-map-sequence-id-365832

    Enlarge

  11. pET24TEV-tSHIP2

    Plasmid
    #183771
    Purpose
    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP2 (INPPL1 Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa18 to aa985 only
    Promoter
    T7 lac
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183771-plasmid-map-sequence-id-362691

    Enlarge

  12. MSNA-c029

    Plasmid
    #175471
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.
    Depositor
    Opher Gileadi
    Article
    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    L281R, H288A
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175471-plasmid-map-sequence-id-353253

    Enlarge

  19. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

    Enlarge

  20. pCW57-RFP-P2A-MCS (Neo)

    Plasmid
    #89182
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89182-plasmid-map-sequence-id-176305

    Enlarge

Showing: 4921 - 4940 of 22169 results