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hSirt3-RFP

Plasmid

#216806

Purpose

Human Sirt3-RFP driven by the CaMKII promoter

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (SIRT3 Human)

Use

Lentiviral

Tags

mRFP1

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM4Di-mCherry

Plasmid

#161579

Purpose

To express hM4Di in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP35_EBOV

Plasmid

#103050

Purpose

Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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CMV BARK p2A mCherry

Plasmid

#117689

Purpose

CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Tags

HA / FLAG

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM3Dq-mCherry

Plasmid

#161578

Purpose

To express hM3Dq in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM3Dq-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pXNgate21-3HA

Plasmid

#105078

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pQTEV-PTD004

Plasmid

#34798

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

GTP-binding protein PTD004 (OLA1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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SEAP (Protein secretion reporter) in pLX317

Plasmid

#233290

Purpose

SEAP reporter (used for monitoring protein secretion)

Depositor

Sefi Rosenbluh

Article

Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

Insert

SEAP

Use

Lentiviral

Expression

Mammalian

Promoter

E1Fa

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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LacZ in pLX304

Plasmid

#42560

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

LacZ

Use

Lentiviral

Tags

2xV5

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc

Plasmid

#176049

Purpose

Expresses Myc tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc396

Plasmid

#161954

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

Plasmid

#159842

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

Plasmid

#159836

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE131

Plasmid

#118485

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE140

Plasmid

#118492

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-PCNT

Plasmid

#227284

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of PCNT (PCNT Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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