We narrowed to 11,112 results for: nar;

1. pBTK569

   Plasmid

   #110614

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V32M (see comments below)

   Promoter

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-Akt1(E17K)

   Plasmid

   #86563

   Purpose

   Expresses human full-length Akt1(E17K) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   E17K mutation in the PH domain

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWP1_mHAL

   Plasmid

   #102318

   Purpose

   expression of mouse cDNA of HAL refractory to the guide in plasmid 4

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Hal (Hal Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB122

   Plasmid

   #68576

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP Rab4DN

   Plasmid

   #99019

   Purpose

   Localization and visualization of the dominant negative form of Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 DN (Rab4 Fly, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   S22N Dominant negative

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CHRFAM7A

   Plasmid

   #62284

   Purpose

   mammalian expression of human duplicated Alpha7 (CHRFAM7A)

   Depositor

   Henry Lester, Sherry Leonard

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial duplication of CHRNA7

   Promoter

   CMV

   Available since

   March 3, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pQZ210

   Plasmid

   #106414

   Purpose

   Co-expresses trans SNARE complex with pDC183 in E.coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Inserts

   Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
   Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Fragment 191-256 , Codon optimization and Fragmen…

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-eYFP-3x-miR708-5p-TS

   Plasmid

   #117381

   Purpose

   An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mNRDc-E>A-V5

   Plasmid

   #51298

   Purpose

   expression of the inactive mutant of mouse NRDc in mammalian cells

   Depositor

   Eiichiro Nishi

   Article

   Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

   Insert

   NRDc E247A (Nrd1 Mouse)
   

   Use

   Tags

   HIS and V5

   Expression

   Mammalian

   Mutation

   E247A, enzymatic inactive mutant

   Promoter

   CMV

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pBTK614

    Plasmid

    #110608

    Purpose

    BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5

    Plasmid

    #161555

    Purpose

    Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMpGWB321

    Plasmid

    #68649

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGWB119

    Plasmid

    #68573

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEM-PH-citrine

    Plasmid

    #131406

    Purpose

    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

    Depositor

    Lars Hufnagel

    Article

    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

    Insert

    PH
    

    Use

    In vitro transcription

    Tags

    citrine yellow fluorescent protein

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-Akt1(D323A/D325A)

    Plasmid

    #86630

    Purpose

    Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1(D323A/D325A) (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDC183

    Plasmid

    #106415

    Purpose

    Co-expresses trans SNARE complex with pQZ210 in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Inserts

    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fragment 141-204, Codon optimization and Fragment…

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGWB121

    Plasmid

    #68575

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFBD-Akt1(T308A/S473A)

    Plasmid

    #86578

    Purpose

    Expresses human full-length Akt1(T308A/S473A) phosphomutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    T308A/S473A phosphomutant

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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