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pLenti-Puro-sgMARK3

Plasmid

#138690

Purpose

Expresses a human MARK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK3 human (MARK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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BPV00605 pET21GG2-His(6)-BECN(1-265)

Plasmid

#136686

Purpose

Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 IDR + CC (BECN1 Human)

Tags

Hexa-Histadine

Expression

Bacterial

Mutation

DNA optimized for E. coli expression

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBiex1-SYNAC

Plasmid

#82710

Purpose

Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

Insert

SYNAC (ADCY2, ADCY5 Human)

Tags

FLAG, mCerulean, and mCitrine

Expression

Bacterial and Insect

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192239

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192242

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARD14-D176H

Plasmid

#69919

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

D176H

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC35D2_STOP

Plasmid

#161288

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D2 (SLC35D2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3-Flag-SPRTN-Y117C

Plasmid

#110216

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

Flag

Expression

Mammalian

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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3294_pETcon-SARS2-RBD_Omicron-BA1

Plasmid

#184408

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHE Short

Plasmid

#137697

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV superYFP-AURKA K162M-mTurq2

Plasmid

#157773

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and superYFP

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHG Short

Plasmid

#137699

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mal*leu*

Plasmid

#21173

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

First point mutation at nucleotide 5114 (numberin…

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-MRTFA-HA

Plasmid

#247436

Purpose

siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

MRTF-A (Mrtfa Mouse)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Mutation

siRNA resistant - no amino acid mutations

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-Rho(G14V)-HA

Plasmid

#247437

Purpose

HA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

RhoA(G14V) (RHOA Human)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Mutation

G14V mutation (constitutive active mutant)

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-SUMO-m-cGAS(I309T)

Plasmid

#232295

Purpose

Expression of mutant mouse cGAS protein (I309T)

Depositor

Thomas Tuschl

Article

Skeldon et al Commun Chem. 2025 Mar 22;8(1):88. doi: 1

Insert

MB21D1 (Cgas Mouse)

Expression

Bacterial

Mutation

I309T

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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Human Wild-type ASAP1

Plasmid

#235212

Purpose

Expresses Wild-Type ASAP1 in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al Proc Natl Acad Sci U S A. 2018 May 1;115

Insert

ASAP1 (ASAP1 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:LRRC10_CE_pISceI

Plasmid

#223042

Purpose

Express LRRC10 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

LRRC10 (LRRC10 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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