We narrowed to 7,663 results for: ski

1. pCS2+HyPer7

   Plasmid

   #136466

   Purpose

   Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-myr-AKT-HA

   Plasmid

   #31789

   Depositor

   Xin Chen

   Article

   Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

   Insert

   Akt1 (Akt1 Mouse)
   

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET32a-hHBEGF

   Plasmid

   #199234

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proheparin-binding EGF-like growth factor (HBEGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLifeAct-HyPer7

   Plasmid

   #136464

   Purpose

   Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   LifeAct

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-sgFSP1

   Plasmid

   #186026

   Purpose

   knock out FSP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Ferroptosis Suppressor Protein 1 (AIFM2 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2-sgACSL4

   Plasmid

   #186024

   Purpose

   knock out ACSL4 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-GINKO2

   Plasmid

   #177116

   Purpose

   Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

   Depositor

   Robert Campbell

   Article

   Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

   Insert

   GINKO2
   

   Tags

   6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2_IRES2_EGFP_KCNQ2 var4

   Plasmid

   #173154

   Purpose

   For mammalian expression of KCNQ2 and creating variants

   Depositor

   Al George

   Article

   Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

   Insert

   KCNQ2 (KCNQ2 Human)
   

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Promoter

   Simian CMV IE94

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

   Plasmid

   #231721

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Tags

   Flag-GST-3C

   Expression

   Insect

   Mutation

   RPB1:1586-1971

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-DIO-ATLASsnFlp

    Plasmid

    #232352

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    ATLASsnFlp
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid

    #106174

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1 and AAV9

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2 sgKEAP1-3

    Plasmid

    #186837

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR v2 sgKEAP1-2

    Plasmid

    #186459

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-DIO-BACE-HA

    Plasmid

    #232353

    Purpose

    Beta-Secretase to be expressed (1:1) with either ATLAS protein.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    BACE (BACE1 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/Ndi-1/myc-His

    Plasmid

    #127503

    Purpose

    Expresses myc-tagged yeast Ndi-1 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    Ndi-1 (NDI1 Budding Yeast)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFEB promoter-luciferase reporter

    Plasmid

    #66801

    Purpose

    Reporter for activity of TFEB gene promoter

    Depositor

    Albert La Spada

    Article

    Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

    Insert

    TFEB promoter (Tfeb Mouse)
    

    Use

    Luciferase

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid

    #188453

    Purpose

    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTB (ACTB Human)
    

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX307 MCL1

    Plasmid

    #117726

    Purpose

    Open reading frame vector encoding MCL1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    MCL1L (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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