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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBSPPc

Plasmid

#116918

Purpose

Gene constitutive expression from a Pc promoter

Depositor

Cuiqing Ma

Publication

Xu et al Appl Environ Microbiol. 2013 Apr;79(8):2

Type

Empty backbone

Expression

Bacterial

Promoter

Pc promoter from Delftia acidovorans C17

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pANY6

Plasmid

#112198

Purpose

This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.

Depositor

Yingfeng An

Publication

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

ELP-tag

Expression

Bacterial

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUX19

Plasmid

#36328

Depositor

Gary Roberts

Type

Empty backbone

Use

Suicide vector for r. rubrum

Expression

Bacterial

Promoter

reduce strength lacZ

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p356_DG

Plasmid

#234699

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM53151

Plasmid

#169093

Purpose

Level 1 cloning vector for transient expression. Already contains the 35S promoter and the Nos terminator.

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ143-ZmUbi

Plasmid

#138107

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB6631

Plasmid

#106161

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6630

Plasmid

#106160

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV210

Plasmid

#87628

Purpose

Drives Bxb1 expression in the D. melanogaster germ line.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1

Expression

Insect

Promoter

vasa

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV601

Plasmid

#87632

Purpose

RMCE Bxb1 attB-flanked vector.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attB

Use

Rmce

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SlugBE4max

Plasmid

#163799

Purpose

Expresses AncBE4max and nSlugCas9

Depositor

Yongming Wang

Publication

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTHSSe_53

Plasmid

#109251

Purpose

pUC BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_51

Plasmid

#109249

Purpose

p15A BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_39

Plasmid

#109238

Purpose

PTac driving TALEsp1

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

TALEsp1

Expression

Bacterial

Promoter

PTac

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pPTn2P-PTK

Plasmid

#85604

Purpose

Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Publication

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Passport transposon with a puromycin-thymidine kinase fusion

Expression

Mammalian

Promoter

PGK

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJG380

Plasmid

#91188

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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PMV-ycrtI

Plasmid

#65342

Purpose

systhesis of crtI gene in the beta-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtI

Use

Synthetic Biology

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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