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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,078 results for: nls

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Showing: 4941 - 4960 of 21078 results

  1. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  2. CRY-Gal∆DD (B1013)

    Plasmid
    #92035
    Purpose
    Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES
    Depositor
    Chandra Tucker
    Publication
    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2 (CRY2 Mustard Weed)
    Expression
    Mammalian
    Mutation
    Fusion of plant CRY2 to Gal4 binding domain (AA1-…
    Available since
    July 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92035-plasmid-map-sequence-id-183927

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  4. pv6_BRD4(1)_BRD.1x_mutant_N140F

    Plasmid
    #260593
    Purpose
    Expresses a binding-deficient mutant of the first bromodomain from BRD4 fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(1)_BRD.1x_mutant_N140F (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    N140F
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260593-plasmid-map-sequence-id-523808

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  5. pv6_BRD4(2)_BRD.1x_mutant_N434F

    Plasmid
    #260594
    Purpose
    Expresses a binding-deficient mutant of the second bromodomain from BRD4 fused to eGFP including flexible linker and NLS
    Depositor
    Tuncay Baubec
    Insert
    BRD4(2)_BRD.1x_mutant_N434F (Brd4 Mouse)
    Expression
    Mammalian
    Mutation
    N434F
    Promoter
    CAGGS
    Available since
    Oct. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    260594-plasmid-map-sequence-id-523753

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  13. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  14. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  15. pCEE

    Plasmid
    #159746
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159746-plasmid-map-sequence-id-313813

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  18. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219823-plasmid-map-sequence-id-441127

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Showing: 4941 - 4960 of 21078 results