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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,742 results for: CAN

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Showing: 2481 - 2490 of 22742 results

  1. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    John Ngo
    Article
    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi
    Insert
    dCas9-NS3-NLS/VPR
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112244-plasmid-map-sequence-id-216918

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  2. pCAP03-ermE*p

    Plasmid
    #120234
    Purpose
    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    Use
    Synthetic Biology
    Mutation
    bla (amp resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120234-plasmid-map-sequence-id-233748

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  3. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  4. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

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  9. mOtop1_pGEMHE

    Plasmid
    #114674
    Purpose
    This construct was used to express mouse Otop1 in Xenopus laevis oocytes
    Depositor
    Emily Liman
    Article
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Insert
    Otopetrin 1 (Otop1 Mouse)
    Use
    For generating the mrna, which can highly express…
    Mutation
    SNP (rs49325632): Glycine 396 to Alanine
    Promoter
    T7
    Available Since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114674-plasmid-map-sequence-id-226716

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Showing: 2481 - 2490 of 22742 results