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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pFRT/TO/FLAG/HA-HDLBP

Plasmid

#154450

Purpose

Expression of FLAG/HA-tagged HDLBP

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

HDLBP (HDLBP Human)

Expression

Mammalian

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-YAPS94A

Plasmid

#84010

Purpose

expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S94A

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX301-ARF6-TagRFP

Plasmid

#162029

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCR-XL-rAkap9

Plasmid

#196867

Purpose

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akap9 (Akap9 Rat)

Expression

Bacterial

Promoter

Lac promoter and T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN2-pLenti6

Plasmid

#36950

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN2 (EGLN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN3-pLenti6

Plasmid

#36951

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN3 (EGLN3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

Plasmid

#149714

Purpose

Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145270

Purpose

Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(53Q)-IDR (TBP Human)

Expression

Mammalian

Mutation

Q-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145269

Purpose

Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

Plasmid

#206956

Purpose

AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

mScarlet-I, MmMT3, QtEnc

Use

AAV

Tags

FLAG

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX303-ARF6-mNeonGreen

Plasmid

#162027

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145261

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL

Plasmid

#160286

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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