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pMpGWB114

Plasmid

#68568

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-BRD3

Plasmid

#23410

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRD3 (BRD3 Human)

Use

Gateway Cloning

Mutation

C1286T

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX4T1-Smurf1 C699A

Plasmid

#13503

Depositor

Jeff Wrana

Publication

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

GST

Expression

Bacterial

Mutation

C699A: catalytically inactive

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-ALK3 K261R

Plasmid

#80875

Purpose

mammalian expression of ALK3 K261R

Depositor

Aristidis Moustakas

Publication

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK3 (BMPR1A Human)

Tags

HA

Expression

Mammalian

Mutation

K261R (Kinase inactive)

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y925F

Plasmid

#50509

Depositor

Kenneth Yamada

Publication

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y925F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Publication

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
GFP-Golgin-84-TEV #709#243

Plasmid

#42108

Depositor

Ayano Satoh

Publication

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Golgin-84 (GOLGA5 Human)

Tags

GFP

Expression

Mammalian

Mutation

inserted ENLYFQG b/w 300 and 301

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA 3.2-FUS-1-413aa-V5

Plasmid

#29612

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FUS-1-413aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-413

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

Plasmid

#67276

Purpose

RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

Depositor

Jannik Elverløv-Jakobsen

Publication

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Inserts

GFP (mWasabi)
hygro

Tags

F3 and Frt

Expression

Mammalian

Promoter

CMV

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC519

Plasmid

#62278

Purpose

Yeast dCas9-VP64 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9-VP64

Tags

Has a VP64 fusion

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Publication

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
yfpsyndat

Plasmid

#19991

Depositor

Jonathan Javitch

Publication

Carvelli et al J Neurochem. 2002 May . 81(4):859-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

yfpsynhdat (SLC6A3 Human)

Tags

yfp

Expression

Mammalian

Available since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-deltaCKAR

Plasmid

#31526

Depositor

Alexandra Newton

Publication

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMRE-Tn7-166

Plasmid

#118578

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Publication

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Publication

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AMPK Biosensor

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSC-PreE2

Plasmid

#52264

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human)

Tags

GST

Expression

Bacterial

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-EPHA6

Plasmid

#23920

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHA6 (EPHA6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-GATA6

Plasmid

#101418

Purpose

Donor Vector containing GATA6 transcription factor, part of the Human TFome Collection

Depositor

George Church

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

GATA6 (GATA6 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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