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pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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PCDNA3-GFP1-9 T2A mCherry

Plasmid

#124430

Purpose

Expresses GFP1-9 and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9 T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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TPC1-G-GECO1.2

Plasmid

#207143

Purpose

Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPCN1 (TPCN1 Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP1-P2A-FusionRed

Plasmid

#188732

Purpose

Express miniGFP1 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP718nano

Plasmid

#195744

Purpose

Monomeric near-infrared fluorescent protein miRFP718nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-FLuc

Plasmid

#188311

Purpose

Expression of Firefly luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Firefly luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-PIK3C2B

Plasmid

#161989

Purpose

Mammalian expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant, Volker Haucke

Article

Marat et al Science. 2017 Jun 2;356(6341):968-972. d

Insert

PIK3C2B (PIK3C2B Human)

Tags

GFP

Expression

Mammalian

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-HaloTag7-CHMP4A

Plasmid

#184895

Purpose

Stable expression of HaloTag7-CHMP4A in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

CHMP4A (CHMP4A Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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FLT3-D835Y-MSCV-IRES-GFP

Plasmid

#184780

Purpose

Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

Depositor

Timothy Ley

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Activating tyrosine kinase domain mutation (D835Y)

Promoter

MSCV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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LOC5567066 Gastrin-releasing peptide receptor

Plasmid

#115372

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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SlugCas9

Plasmid

#163793

Purpose

Expresses SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA7-mRuby3

Plasmid

#184893

Purpose

Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA7 (ANXA7 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NAIP5

Plasmid

#60205

Purpose

Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

Depositor

Russell Vance

Article

Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

Insert

NAIP5

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-VP64_C-term

Plasmid

#136887

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the VP64 activator domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

VP64 activator domain

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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