We narrowed to 9,299 results for: yeast expression

1. pABD823

   Plasmid

   #67911

   Purpose

   Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae

   Depositor

   Bruce Downie

   Article

   Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

   Insert

   Cold temperature germinating 10 (CTG10)
   

   Use

   Tags

   GAL4 DNA binding domain (148 amino acids)

   Expression

   Yeast

   Mutation

   Promoter

   pADH1 (alcohol dehydrogenase 1 promoter)

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

2. TEF2pro_mCherry_YFP

   Plasmid

   #138623

   Purpose

   Dual reporter backbone plasmid for CCRA experiment

   Depositor

   Rob Mitra

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TEF1pro_mCherry_YFP

   Plasmid

   #138624

   Purpose

   Dual reporter backbone plasmid for CCRA experiment

   Depositor

   Rob Mitra

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCC001

   Plasmid

   #124451

   Purpose

   Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   HH-BsaI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   gRNA expression cassette from pUDP002 modified to…

   Promoter

   TDH3 promoter from S.cerevisiae (already present …

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Use

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIV489

   Plasmid

   #177697

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIV490

   Plasmid

   #177698

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYea5.1_Notch1-L24_sfGFP

   Plasmid

   #207865

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYea5.1_C99_sfGFP

    Plasmid

    #207802

    Purpose

    Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-C100-sfGFP-GAL4 fusion protein
    

    Use

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYea5.1_Notch3_sfGFP

    Plasmid

    #207864

    Purpose

    Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein
    

    Use

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYea5.1_Notch1_sfGFP

    Plasmid

    #207801

    Purpose

    Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein
    

    Use

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193318

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Use

    Tags

    4MYC

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193317

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Use

    Tags

    4FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV492

    Plasmid

    #177700

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDIV491

    Plasmid

    #177699

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDIV488

    Plasmid

    #177696

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Coexpressed Free ClLIS1 (M)

    Plasmid

    #182484

    Purpose

    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS(M)
    LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT1219

    Plasmid

    #228301

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    GGG linker followed by His6 and MF_ secretion sig…

    Expression

    Yeast

    Mutation

    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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