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Arp3-TagRFP

Plasmid

#195091

Purpose

Gene Overexpression of Human Arp3

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

Arp3 (APOBEC3A Human)

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S178A

Plasmid

#195097

Purpose

Gene Overexpression of Human SHARPINS178A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S178A

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S131A

Plasmid

#195095

Purpose

Gene Overexpression of Human SHARPINS131A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S131A

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S146E

Plasmid

#195099

Purpose

Gene Overexpression of Human SHARPIN S146E

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S146E

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S285A

Plasmid

#195098

Purpose

Gene Overexpression of Human SHARPINS285A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S285A

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S146A

Plasmid

#195096

Purpose

Gene Overexpression of Human SHARPINS146A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S146A

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SHARPIN L276A

Plasmid

#195093

Purpose

Gene Overexpression of Human SHARPIN L276A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

L276A

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC

Plasmid

#116992

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0361_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_3

Plasmid

#116973

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3

Plasmid

#116991

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSK

Plasmid

#136552

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OKM

Plasmid

#136554

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSM

Plasmid

#136555

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-E2A-cMyc (Myc, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00320

Plasmid

#34445

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6376 (E2f1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pPOU5F1-donor

Plasmid

#176367

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

POU5F1 (POU5F1 Human)

Use

CRISPR

Tags

GFP

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pOTF3-AK (clone3)

Plasmid

#21165

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

POUF5F1 (genomic DNA) (POU5F1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

NeoR, EGFP, and SV40 polyA are between the two ge…

Available Since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3-myc

Plasmid

#11813

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 (CASP3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-mMALAT1_3' Sense

Plasmid

#60647

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-bGH pA Sense

Plasmid

#60645

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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