We narrowed to 54,602 results for: Ran

1. pcDNA-Ran-T24N-mRFP1-polyA

   Plasmid

   #104561

   Purpose

   Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   T24N

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0044-EF1a-RanCas13b-NES-mapk

   Plasmid

   #103855

   Purpose

   Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   RanCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and mapk NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52379

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   OCT4

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.23-IL2RA CaRE4 (plus strand)

   Plasmid

   #91850

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0345)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23-IL2RA CaRE4 (minus strand)

   Plasmid

   #91849

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0626)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Orange Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71346

   Purpose

   Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. BCR/ABL P190 transgenic construct

   Plasmid

   #38185

   Depositor

   Nora Heisterkamp

   Article

   Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

   Insert

   BCR/ABL P190 (ABL1, BCR Human)
   

   Use

   Construct for making transgenic mice

   Tags

   Expression

   Mutation

   Promoter

   truncated mouse MT promoter

   Available since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET co-transformation cloning vector (13S-A)

    Plasmid

    #48323

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23-IL2RA CaRE1 (plus strand)

    Plasmid

    #91837

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0614)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pColdII-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid

    #75355

    Purpose

    Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

    Depositor

    Kalle Gehring

    Insert

    Sacsin Xenopus (Silurana) tropicalis 87-1376
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

    Plasmid

    #149693

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRY2clust-mCherry-RANK-P2A-CIBNcaax

    Plasmid

    #208613

    Purpose

    Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

    Depositor

    Takao Nakata, Tomohiro Ishii

    Article

    Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

    Insert

    CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)
    

    Use

    Tags

    CAAX and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

    Plasmid

    #118000

    Purpose

    Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    Derlin1-mOrange2
    

    Use

    Plant/bacteria binary vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin-10

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. P-miR-145 (pTransluc-miR-145-1)

    Plasmid

    #21496

    Depositor

    Kenneth Kosik

    Article

    Xu et al Cell. 2009 May 15. 137(4):647-58.

    Insert

    1.5kb 5' of miR-145 (MIR145 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nano-lantern-plasma membrane/pcDNA3

    Plasmid

    #51975

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    HIS and Lyn

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGL4.23-CD69 CaRE3 (plus strand)

    Plasmid

    #91844

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0621)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (plus strand) (CD69 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT-ubb-Avi-Cerulean-RanGAP

    Plasmid

    #79881

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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