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Plasmid#195960PurposeGateway pME fluorophoreDepositorInsertmApple
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAS12124_PEAR-GFP-preedited
Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-G_oT7pol-IRES-BSD
Plasmid#172369PurposeGeneration of oT7pol-expressing stable cell lines, resistant to the antibiotic BlasticidinDepositorInsertoT7pol + BSD
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-SADB19_oG-IRES-Puro
Plasmid#172367PurposeGeneration of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic PuromycinDepositorInsertoG + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
460 p3E-2A-mCherrypA
Plasmid#195970PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCherry
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9-H840A
Plasmid#80453PurposeSpCas9 mammalian expression vectorDepositorInsertSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
UseTags8xHisExpressionBacterialMutationPromoterPBAD30Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(0)
Plasmid#164269PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLA CMV N-HA RALB WT
Plasmid#50989PurposeExpresses RALB in mammalian cellsDepositorInsertRALB (RALB Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB4k5_RNAnano (r-plasmid)
Plasmid#127634PurposeEncodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.DepositorInsertRNA nanostrcutre
UseSynthetic BiologyTagsExpressionMutationPromoterT7 RNAPAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pENTR4 AvrRpm1
Plasmid#209185PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tagsDepositorInsertAvrRpm1
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4E02
Plasmid#71024PurposeGateway entry cloneDepositorInsertsimj (simj Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBITE-CD19-CD3
Plasmid#190677PurposeEncodes a modularized form of HD37-OKT3 bispecific antibodyDepositorInsertModularized-HD37-modularized-OKT3-P2A-NeonGreen
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F269-OKT3
Plasmid#190681PurposeEncodes an EGFRvIII-CD3 BITE protein (F269 clone)DepositorInsertAnti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only