Addgene

We narrowed to 5,346 results for: aaas

Showing: 481 - 500 of 5346 results

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pGALSc104b F130W

Plasmid

#1309

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Phe 130 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y257W

Plasmid

#1310

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 257 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y466W

Plasmid

#1311

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y466W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 466 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y507W

Plasmid

#1312

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 507 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b L553W

Plasmid

#1313

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Leu 553 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y819W

Plasmid

#1314

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 819 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b L892W

Plasmid

#1315

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Leu 892 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4

Plasmid

#167542

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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KBB394

Plasmid

#185086

Purpose

NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

Nma111 KKR 9-11 AAA; KRK 28-30 AAA

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Mdn1-FL

Plasmid

#171602

Purpose

Expresses full length S. pombe Mdn1 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Use

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Promoter

Available since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330) S278C

Plasmid

#87739

Purpose

Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-277, mutated Ser 278 to Cys

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(81-437)

Plasmid

#87734

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-80

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge