We narrowed to 7,713 results for: pCas

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pCAG-AILR-LplA-ER

Plasmid

#42574

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LplA(AILR) with ER-retention sequence KDEL

Tags

FLAG

Mutation

Compared to wild-type LplA: tryptophan 37 to alan…

Promoter

CAG

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG Fzd7 GFP

Plasmid

#66106

Purpose

expression of chicken Fzd7 GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

Fzd7 (FZD7 Chicken)

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
pCaSpeR-act5cB-QF#7m1

Plasmid

#46126

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Expression

Insect

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-LSS-mOrange

Plasmid

#89688

Purpose

pCAG promoter driving LSS-mOrange

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

LSS-mOrange

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dHeFSpCas9

Plasmid

#92116

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBW829_pCAG-pExpr-LUT

Plasmid

#87554

Purpose

Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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CpcB-Solanum-lycopersicumPHLS_CpcA

Plasmid

#74003

Purpose

Expresses the Solanum lycopersicum PHLS as a fusion with the CpcB protein plus the NPPS and CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Inserts

PHLS and CmR
Neryl diphosphate synthase from Solanum lycopersicum

Promoter

cpc

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–EgRNA

Plasmid

#62716

Purpose

Empty; no U6 gRNA cassette

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-Trex2-bpA

Plasmid

#86984

Purpose

Expression of mouse Trex2

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

mouse Trex2 coding region (Trex2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hCD4-GCaMP2

Plasmid

#18932

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

hCD4-GCaMP2 (CD4 Human)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-EGFP

Plasmid

#79407

Purpose

Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-HDAC4 C-terminus

Plasmid

#71400

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 C-terminus (HDAC4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-B-838;+80pCalb2

Plasmid

#66750

Purpose

luciferase reporter for Calb2 promoter (-838 to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-838bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBT225_(pCA-tdT3Myc)

Plasmid

#36873

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Tags

3 Myc tags

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4 1-747

Plasmid

#71399

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 1-747 (HDAC4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-Intron4-PAFAH1B1

Plasmid

#92434

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only