We narrowed to 9,861 results for: plasmids 101

1. MIGR-mFoxP3

   Plasmid

   #24067

   Depositor

   Dan Littman

   Insert

   Foxp3 (Foxp3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. rk17s

   Plasmid

   #117437

   Purpose

   Expression of ARL14

   Depositor

   Richard Kahn

   Insert

   ARL14 (ARL14 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124287

   Purpose

   sgRNA against human Col7A1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

4. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124286

   Purpose

   sgRNA against human Col7A1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2iD6Hoxa13bER4(#686)

   Plasmid

   #184101

   Purpose

   cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   ZfHoxa13b-ERT2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKP2032

   Plasmid

   #167651

   Purpose

   Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A

   Depositor

   Kathleen Postle

   Insert

   his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S1…

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. INT_SS9sp

   Plasmid

   #212145

   Purpose

   Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

   Depositor

   Kang Zhou

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23119

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-Casp1-His

   Plasmid

   #11809

   Depositor

   Guy Salvesen

   Insert

   Caspase-1 (CASP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLX-PGK-hCDK4

   Plasmid

   #114314

   Purpose

   Constitutive expression of the CDK4 protein

   Depositor

   Patrick Salmon

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnEA-NpM-HsTOB1_Q

    Plasmid

    #147315

    Purpose

    Bacterial Expression of HsTOB1

    Depositor

    Elisa Izaurralde

    Insert

    HsTOB1 (TOB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one non silent mutation F309L compared to the seq…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnEA-NpM-HsTOB1_1-138_Q

    Plasmid

    #147316

    Purpose

    Bacterial Expression of HsTOB1_1-138

    Depositor

    Elisa Izaurralde

    Insert

    HsTOB1_1-138 (TOB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation compared to teh sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLNHA-C1-HsTOB1_Q

    Plasmid

    #147286

    Purpose

    Mammalian Expression of HsTOB1

    Depositor

    Elisa Izaurralde

    Insert

    HsTOB1 (TOB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent and one non silent S228P mutation comp…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC324

    Plasmid

    #110165

    Purpose

    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    R61K change made in ERK-KTR CDS

    Promoter

    lin-31 promoter/enhancer

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. INT_GFPsp

    Plasmid

    #212144

    Purpose

    Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23119

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. INT_GFPsp_CamRcargo

    Plasmid

    #212143

    Purpose

    Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23119

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDAX-554 N-Tag GFP[1] L10 Stat3 EBPF2

    Plasmid

    #130101

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid

    #189554

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBA324

    Plasmid

    #214741

    Purpose

    Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSV015

    Plasmid

    #214738

    Purpose

    Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRW402-P1int-BXB1

    Plasmid

    #235979

    Purpose

    Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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