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Plasmid#178994PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)
Plasmid#178993PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)
Plasmid#178992PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)
Plasmid#178991PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)
Plasmid#178990PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP88
Plasmid#83544PurposeUsed to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.DepositorInsertRPL15A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL15AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP77
Plasmid#83538PurposeUsed to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.DepositorInsertRPL8B promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL8BAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTL-c
Plasmid#81098PurposeGene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertyemCFP
UseCre/LoxTagsyemCFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-c
Plasmid#81108PurposeGene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertyemCFP
UseCre/LoxTagsyemCFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH3
Plasmid#83553PurposeUsed to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.DepositorInsertHXT7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterHXT7Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH0
Plasmid#83552PurposeUsed to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.DepositorInsertTPS1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTPS1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP90
Plasmid#83547PurposeUsed to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.DepositorInsertTEF2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF2Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP82
Plasmid#83541PurposeUsed to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.DepositorInsertYEF3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterYEF3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP80
Plasmid#83540PurposeUsed to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.DepositorInsertRPL3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP78
Plasmid#83539PurposeUsed to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.DepositorInsertSSB1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSB1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-HIS3MX6
Plasmid#129100PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 upGFP
Plasmid#117899Purposeprotein expressionDepositorInsertAtCOPT1 upGFP (COPT1 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT6 upGFP
Plasmid#117903Purposeprotein expressionDepositorInsertAtCOPT6 upGFP (COPT6 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT2 upGFP
Plasmid#117901Purposeprotein expressionDepositorInsertAtCOPT2 upGFP (COPT2 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 uPIC
Plasmid#117898Purposeprotein expressionDepositorInsertAtCOPT1 uPIC (COPT1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only