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pDD282

Plasmid

#66823

Purpose

GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

GFP-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized GFP

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMCRi

Plasmid

#207524

Purpose

Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Use

Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

Plasmid

#67276

Purpose

RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Inserts

GFP (mWasabi)
hygro

Tags

F3 and Frt

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pIreSneo-FLAG/HA Ago3

Plasmid

#10823

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 3 (AGO3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

1 silent mutation

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSIMcpf1

Plasmid

#153034

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304 GFP

Plasmid

#193687

Purpose

Constitutive lentiviral expression of GFP

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

GFP

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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