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pTK_Sox10_enh_87_Cerulean

Plasmid

#130586

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_87

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_85_Citrine

Plasmid

#130585

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_85

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGGF008

Plasmid

#48848

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::BastaR::tNOS

Use

Golden gate compatible cloning vector

Mutation

chi sequence in BastaR removed by silent substitu…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pICH42222

Plasmid

#50328

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation; See De…

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pILGFP4I

Plasmid

#104504

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4Q

Plasmid

#104512

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLY108

Plasmid

#130938

Purpose

Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP4L

Plasmid

#104507

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Nov. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pICH78133

Plasmid

#50292

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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XFIVP Smad3

Plasmid

#14826

Depositor

Rik Derynck

Article

Feng et al Genes Dev. 1998 Jul 15. 12(14):2153-63.

Insert

Smad3 (SMAD3 Human)

Tags

VP16 activation domain

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pET-32M human DKK1c (183–266)

Plasmid

#132626

Purpose

expresses human DKK1c (183–266) in E. coli

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

DKK1 (183–266)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEPuro-rnSOX9

Plasmid

#63009

Purpose

Expresses rat SOX9

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Retroviral

Tags

HA

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pILGFP4H

Plasmid

#104503

Purpose

Used to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SaGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4J

Plasmid

#104505

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4N

Plasmid

#104509

Purpose

Used to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SbGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4R

Plasmid

#104513

Purpose

Used to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SuGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pICH44300

Plasmid

#50340

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTK_FoxD3_enh_372_Citrine

Plasmid

#130583

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_enh_372

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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