We narrowed to 11,532 results for: 110

1. pDEST27-GST-ATXN1 FL(92Q)-S776D

   Plasmid

   #21757

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 92 Q(Glutamines) and…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. integrin beta6 777T pcDNA1 neo

   Plasmid

   #13582

   Depositor

   Dean Sheppard

   Article

   Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

   Insert

   integrin beta 6 777T (ITGB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   777T. Lacks last 11 amino acids of cytoplasmic do…

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

   Plasmid

   #166801

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   S730A

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GSG1l-GFP KI

   Plasmid

   #131492

   Purpose

   Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gsg1l Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN1 VAMP2 R125TAG

   Plasmid

   #69877

   Purpose

   express in mammalian cells for unnatural amino acid incorporation into VAMP2

   Depositor

   Silvio Rizzoli

   Article

   Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Amber and Ochre stop codons in the former linker …

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. LIC-Z-YFP

   Plasmid

   #153544

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   Venus YFP

   Expression

   Mammalian

   Mutation

   with mCherry in LIC-Z replaced by YFP

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST27-GST-ATXN1 FL(30Q)-S776D

   Plasmid

   #21755

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PIK3CD gRNA (BRDN0001146545)

   Plasmid

   #76165

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3CD

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3CD (PIK3CD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT-TODestFLAGHA_RBPMS

   Plasmid

   #59388

   Purpose

   Destination vector with RBPMS for stable cell line generation

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMMP-NGFR:LMP1-IRES-mRFP

    Plasmid

    #36974

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Insert

    NGFR:LMP1 (NGFR, LMP-1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NGFR:LMP1 chimeric receptor consists of aa 1–276 …

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-TetR-tagRFP-T

    Plasmid

    #103809

    Purpose

    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIBN-EYFP-TetR

    Plasmid

    #103798

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-TetR (CIB1, tetR Mustard Weed, E. coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mIFP-Actin-C-18

    Plasmid

    #56211

    Purpose

    Localization: Actin, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    NM_001101.3 (ACTB Human)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128338

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna3 (Chrna3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

    Plasmid

    #166796

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    KK93EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

    Plasmid

    #166800

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    E597R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1Xpress-BcrGAP R1090A

    Plasmid

    #38176

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
    

    Use

    Tags

    Xpress

    Expression

    Mammalian

    Mutation

    aa 1004-1271 with R1090A point mutation

    Promoter

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128337

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna2 (Chrna2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge