We narrowed to 7,649 results for: Pol
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Plasmid#177897PurposeBaculo expression of GST tag fused to SNAP tag and human Shp2DepositorInsertShp2 (PTPN11 Human)
TagsTEV-GST-PreScission cut site-SNAP tagExpressionInsectPromoterPolyhedrinAvailable SinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC8 H143A
Plasmid#224344PurposeExpresses human KDAC8 (HDAC8) H143A in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionInsectMutationHistidine 143 mutated to alaninePromoterPolyhedrinAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
WT USP7 TRAF
Plasmid#227226PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 62-205PromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-GM55
Plasmid#217413PurposeLuciferase expression vector with the minimal GM-CSF promoterDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseExpressionMammalianPromoterHuman -55 to +28 minimal CSF2 promoterAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOET5 GII.4 Sydney noro-VLP
Plasmid#218092PurposeForms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).DepositorInsertnoro-VLP
ExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CDC20:161-477
Plasmid#210943PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.DepositorInsertCDC20:S161-P477 (CDC20 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
BirA*-HA-NES
Plasmid#214473PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.DepositorInsertBirA(R118G)
UseGateway Cloning and LentiviralTagsHA tag and NES (Nuclear Exclusion Sequence)ExpressionMammalianMutationR118GPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_RACK1:1-317
Plasmid#210889PurposeInsect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRACK1:M1-R317 (RACK1 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBactin-Akna-mOrange2
Plasmid#196865PurposeExpression of the centrosomal protein Akna fused to mOrange2DepositorInsertAkna-mOrange2 (Akna Mouse)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
DCAF1:PBC020-D05:C246337
Plasmid#183974PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertDCAF1:PBC020-D05:C246337 (DCAF1 Human)
UseBaculovirus expresssionTags6xHis-TEV and MHHHHHHSSGRENLYFQGPromoterPolyhedrinAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
TagsmRFPExpressionPlantPromoterRD21A nativeAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q62C-A140C
Plasmid#162581PurposeExpresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationGlutamine 62 changed to Cysteine and Alanine 140 …PromoterpolyhedringAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ206
Plasmid#162679PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
TagsHisExpressionBacterialMutationE226T; codon optimized for expression in E. coli …PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF13A tail 361-1749
Plasmid#162720PurposeMammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residuesDepositorInsertKif 13A (Kif13a Mouse)
ExpressionMammalianMutationWT with deletion of the 360 N-terminal residues.PromoterChicken Beta actinAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC4B-His-TEV-TAK1-TAB1
Plasmid#154874PurposeInsect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91DepositorInsertTAK1-TAB1 (TAB1 Human)
TagsLIC TEV HisExpressionInsectMutationCodon optimizedPromoterpolyhedrinAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRK2A I12_pECIA2
Plasmid#114987PurposeBait vector PRK2A I12_pECIA2 should be used with prey vector PRK2A I12_pECIA14.DepositorInsertAT2G07040 (PRK2A Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD8
Plasmid#112820PurposepSMF2 with 8 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD10
Plasmid#112821PurposepSMF2 with 10 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACVR1 (ACVR1Z-c041)
Plasmid#110269PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only