We narrowed to 19,244 results for: Tec

Showing: 4981 - 5000 of 19244 results

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pcDNA3 Flag-MBD1-14-mVenusC

Plasmid

#191393

Purpose

MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1-14-mVenusC

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Id2 Ngfr

Plasmid

#80131

Purpose

overexpression of ID2

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Id2 (Id2 Mouse)

Use

Retroviral

Tags

IRES-Ngfr

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L3-NF-L1-dMb2Cas12a

Plasmid

#177686

Purpose

Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

2xFokI-dMb2Cas12a

Use

CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR (GB2566)

Plasmid

#160595

Purpose

Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-FluB-NP

Plasmid

#165093

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza B virus(B/Georgia/05/2019) nucleocapsid protein

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHBintN

Plasmid

#48135

Purpose

Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector

Depositor

Dieter Jahn

Article

Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCoofy48

Plasmid

#55186

Purpose

Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Single Cassette Destination Vector (pAN2414)

Plasmid

#194220

Purpose

Backbone for constructing single casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTS2386-Tier1-PhCMV-GCaMP6s

Plasmid

#169519

Purpose

Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBT276

Plasmid

#122607

Purpose

express CDA1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1-BE4max

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTK332

Plasmid

#59573

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pShoCAST-sgRNA_entry (BO1)

Plasmid

#181786

Purpose

Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12aD156R

Plasmid

#164659

Purpose

Bacterial expression of mutated LbCas12aD156R using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a-D156R

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Mutation

changed Aspartic Acid 156 to Arginine

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR

Plasmid

#118464

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CP1249-CBEmax

Plasmid

#119804

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1249) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBK-EE-BE3

Plasmid

#85175

Purpose

Expresses EE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

EE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMT462

Plasmid

#139393

Purpose

XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs

Use

Synthetic Biology

Promoter

aTC and BA inducible promoters

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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p35S-PBE

Plasmid

#119772

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

NLS-APOBEC1-nCas9-UGI-NLS

Tags

NLS

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pXNubWtgate

Plasmid

#105080

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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