We narrowed to 26,536 results for: emb

1. pAc5.1B-EGFP-DmeIF4E

   Plasmid

   #79258

   Purpose

   Expresses EGFP-tagged DmeIF4E in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   eIF4E (eIF4E1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   EGFP

   Promoter

   Ac5 promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NET23 pEGFP-N2 (1174)

   Plasmid

   #62037

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NET23 (STING1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. STT3A pmRFP (663)

   Plasmid

   #61997

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

   Plasmid

   #105814

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTH752-CEN-RLuc/min103maxCFLuc

   Plasmid

   #38218

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first 103 codons of the original FLuc gene we…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTH751-CEN-RLuc/min53maxCFLuc

   Plasmid

   #38217

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first 53 codons of the original FLuc gene wer…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTH748-CEN-RLuc/min8maxCFLuc

   Plasmid

   #38214

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first eight codons of the original FLuc gene …

   Promoter

   ADH1 and TDH3 (=GDP)

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH753-CEN-RLuc/min346maxCFLuc

   Plasmid

   #38219

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first 346 codons of the original FLuc gene we…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH747-CEN-RLuc/min4maxCFLuc

   Plasmid

   #38213

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The first four codons of the original FLuc gene w…

   Promoter

   ADH1 and TDH3 (=GDP)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid

    #132524

    Purpose

    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNC2H1 (DYNC2H1 Human)
    

    Tags

    8x His, Linker-TEV-linker-TEV, Precission cleavag…

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid

    #102421

    Purpose

    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    Tet1 (Tet1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Modified at the Dharmacon SMARTpool siRNA #2 targ…

    Promoter

    TetO-CMV

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid

    #50486

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    GFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the GFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3.1-nAChr-beta3-YFP

    Plasmid

    #50484

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP fusion in M3-M4 loop (after residue P379). O…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid

    #105805

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Venus

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid

    #105803

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-FLAG; ORF2: TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid

    #50483

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    YFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the YFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1-nAChr-beta3-CFP

    Plasmid

    #50485

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP fusion in M3-M4 loop (after residue P379). O…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid

    #50482

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    CFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the CFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only