We narrowed to 7,663 results for: ski

1. pFRT-FLAG-HA-(N)-CDK12

   Plasmid

   #231750

   Purpose

   Expression in Flp-IN human cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   CDK12 (CDK12 Human)
   

   Tags

   Flag-HA-3C

   Expression

   Mammalian

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human SHIP1 (clone hp51CN)

   Plasmid

   #123347

   Purpose

   Expression of human SHIP1

   Depositor

   Christophe Erneux

   Article

   Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

   Insert

   INPP5D (INPP5D Human)
   

   Expression

   Mammalian

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE HA Chk2 KD, dominant negative chk2

   Plasmid

   #41902

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   Chk2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D347A

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1 _Gata6

   Plasmid

   #73537

   Purpose

   Inducible lentiviral expression of Gata6

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Gata6 (Gata6 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-CAG-AtAFB2.F74A-IRES-puro

   Plasmid

   #216245

   Purpose

   Express AtAFB2.F74A mutant without tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABEpuro-gateway

   Plasmid

   #51070

   Purpose

   gateway adapted mammalian expression vector

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIBN-EYFP-TetR

   Plasmid

   #103798

   Purpose

   BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-TetR (CIB1, tetR Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139090

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-hEGF

   Plasmid

   #199231

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Epidermal growth factor, partial (EGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid

    #182532

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    hM4Di-mCherry

    Expression

    Mammalian

    Promoter

    tetO promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid

    #183765

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    ChR2-EYFP

    Expression

    Mammalian

    Promoter

    tetO promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid

    #225617

    Purpose

    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

    Plasmid

    #192184

    Purpose

    Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 6 heavy chain (HH06)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

    Plasmid

    #192183

    Purpose

    Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIBN-EYFP-hPMLIII

    Plasmid

    #103808

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgGFP_3

    Plasmid

    #78165

    Purpose

    sgGFP

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMarsL274G

    Plasmid

    #63177

    Purpose

    This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    MarsL274G (Mars1 Mouse)
    

    Expression

    Mammalian

    Mutation

    L274G

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Emt-SH2

    Plasmid

    #46424

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Emt (ITK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 223-346

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYX233-DHFR

    Plasmid

    #163761

    Purpose

    Gal-inducible yeast expression of cytosolic DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    DHFR (Dhfr Mouse)
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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