Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 13,849 results for: 158

Showing: 7501 - 7530 of 13849 results

  1. pMCh-1474

    Plasmid
    #82420
    Purpose
    Plasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82420-plasmid-map-sequence-id-156264

    Enlarge

  2. pMCh-1475

    Plasmid
    #82421
    Purpose
    Plasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-1158 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82421-plasmid-map-sequence-id-156262

    Enlarge

  3. pMCh-1476

    Plasmid
    #82422
    Purpose
    Plasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82422-plasmid-map-sequence-id-156258

    Enlarge

  4. pMCh-1477

    Plasmid
    #82423
    Purpose
    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-1158 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82423-plasmid-map-sequence-id-156255

    Enlarge

  5. pMCh-1479

    Plasmid
    #82425
    Purpose
    Plasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82425-plasmid-map-sequence-id-156246

    Enlarge

  6. pMCh-1480

    Plasmid
    #82426
    Purpose
    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82426-plasmid-map-sequence-id-156240

    Enlarge

  9. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

    Enlarge

  10. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

    Enlarge

  11. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

    Enlarge

  12. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

    Enlarge

  13. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

    Enlarge

  14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

    Enlarge

Showing: 7501 - 7530 of 13849 results