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pmMaple3-CAM

Plasmid

#101148

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple3

Expression

Bacterial

Promoter

pBAD

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-mCherry

Plasmid

#66534

Purpose

Expresses mCherry under the control of the Concensus T7 Promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Consensus T7

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-LaminB1-10

Plasmid

#58020

Purpose

Localization: Nuclear Envelope, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

Plasmid

#203908

Purpose

Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4UAS_dGFP_PGK_iRFP

Expression

Mammalian

Promoter

5xUAS and PGK

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCD185

Plasmid

#113317

Purpose

dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRS402-GPD-GFP(S65T)

Plasmid

#85190

Purpose

Integrating plasmid, GFP(S65T) under GPD promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

EGFP

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

GPD

Available Since

Dec. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN G129E

Plasmid

#50523

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

G129E

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-deltaHeat

Plasmid

#25068

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains the LRR-ROC-COR-Kinase-WD40 domains; Del…

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pBTBX-1

Plasmid

#26067

Depositor

Ryan Gill

Article

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pBTBXh-4

Plasmid

#26080

Depositor

Ryan Gill

Article

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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SNRPA_Full-length_pGEX

Plasmid

#135134

Purpose

Recombinant expression of SNRPA with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SNRPA (SNRPA Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Feb. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Tom7

Plasmid

#66571

Purpose

Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

PhyB (PHYB Mustard Weed)

Tags

Tom7 and mCherry

Expression

Yeast

Mutation

PhyB portion contains aa's 1-908

Promoter

pADH1

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CMV10-Flag-mMcl-1 KR

Plasmid

#32979

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Tags

3XFlag

Expression

Mammalian

Mutation

All lys changed to Arg

Promoter

CMV

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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pKT0210

Plasmid

#8724

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD(V203D)

Plasmid

#190090

Purpose

Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD(V203D)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEF073 MBP-Axin-His

Plasmid

#154011

Purpose

Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Mumps V-FLAG

Plasmid

#44908

Depositor

Curt Horvath

Article

Ulane et al J Virol. 2003 Jun;77(11):6385-93.

Insert

Mumps V (V/P )

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only
TLS 1: hTLS.pBSKS(+)

Plasmid

#21827

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Expression

Bacterial

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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