167,318 results

1. Fibronectin-human-plasma in pMAX

   Plasmid

   #120401

   Purpose

   enables eukaryotic expression of human plasma fibronectin

   Depositor

   Inaam Nakchbandi

   Article

   Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

   Insert

   Fibronectin (FN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJR022

   Plasmid

   #198820

   Purpose

   C. elegans optimized version of mScarlet

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::wrmScarlet::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-His-NDK

   Plasmid

   #124136

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Nucleotide diphosphate kinase (ndk Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Bxb1-LP-v2-TC

   Plasmid

   #194326

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mKeima-Red-Mito-7

   Plasmid

   #56018

   Purpose

   Localization: Mitochondria, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Tags

   mKeima-Red

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Expression

   Bacterial

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. hCathepsin B

    Plasmid

    #11249

    Purpose

    Mammalian expression of human cathepsin B

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin B (CTSB Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. M23: pHR-SFFVp-FUSN::mCherry::SspB

    Plasmid

    #122148

    Purpose

    N terminal domain of FUS (1-214) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6/V5-p53_wt p53

    Plasmid

    #22945

    Purpose

    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMMP-TVA800

    Plasmid

    #15778

    Depositor

    Edward Callaway

    Article

    Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

    Insert

    TV A800
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-mCherry (AAV9)

    Viral Prep

    #114469-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

18. mito-dendra2

    Plasmid

    #55796

    Purpose

    photo-activatable mitochondrial marker for expression in mammalian cells

    Depositor

    David Chan

    Article

    Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

    Insert

    mito-Dendra2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-hSyn-Soma-jGCaMP8s (AAV9)

    Viral Prep

    #169256-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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