We narrowed to 11,769 results for: 110

1. pSICO-CPSF6-iRFP670

   Plasmid

   #167586

   Purpose

   Expresses human CPSF6 fused to iRFP

   Depositor

   Zandrea Ambrose

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   CPSF6-iRFP670 (CPSF6 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Mutation

   WT

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-H1-shRNA-CMV-HA-PACSIN1

   Plasmid

   #72577

   Purpose

   Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

   Depositor

   Richard Huganir

   Article

   Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

   Inserts

   PACSIN1 (Pacsin1 Mouse)
   H1-PACSIN1 shRNA cassette
   

   Use

   RNAi

   Tags

   HA

   Expression

   Mammalian

   Mutation

   5 silent mutations around shRNA recognition seque…

   Promoter

   CMV and H1

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human_TFAM_NoMTS_L6_pET28a+

   Plasmid

   #60012

   Purpose

   Expresses TFAM No MTS L6 mutant in bacterial cells

   Depositor

   David Chan

   Article

   Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

   Insert

   Human TFAM No MTS (TFAM Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Missing 1st 42 aas (Mitochondrial targeting seque…

   Promoter

   T7

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-tdPCP-CIBN

   Plasmid

   #183937

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-CIBN (CIB1 Synthetic, Mustard Weed)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK2-E2F3 3'UTR

   Plasmid

   #29470

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F3 3'UTR (E2F3 Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pEBTet-SNAP-γ-tubulin

   Plasmid

   #136799

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-TUBG (TUBG1 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-neo-myc-LC3(deltaC22, G120A)

   Plasmid

   #45449

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Encodes amino acids 1-120, Glycine at position 12…

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pTER GAMMA-TUBULIN shRNA Human

   Plasmid

   #87955

   Purpose

   reduced the expression of gamma-tubulin in human cells

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   sh gamma-tubulin (TUBG1 Human)
   

   Tags

   No-tag

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene.…

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-hu-c-Myc-dN2

   Plasmid

   #50774

   Purpose

   retroviral expression of human c-Myc dN2 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN2 (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 42-439

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-hu-c-Myc-dN1

    Plasmid

    #50773

    Purpose

    retroviral expression of human c-Myc dN1 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc -dN1 (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 14-439

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1068 pGL3 FasL promoter (no SV40 prom)

    Plasmid

    #9029

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Removed the SV40 promoter from pGL3

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-6P-1-INTS3-C

    Plasmid

    #128417

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    INTS3 C-terminal region (514-1042 aa)

    Promoter

    Tac promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

14. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

    Plasmid

    #166801

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    S730A

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE GSG1l-GFP KI

    Plasmid

    #131492

    Purpose

    Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gsg1l Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pN1 VAMP2 R125TAG

    Plasmid

    #69877

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    Amber and Ochre stop codons in the former linker …

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

18. LIC-Z-YFP

    Plasmid

    #153544

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Tags

    Venus YFP

    Expression

    Mammalian

    Mutation

    with mCherry in LIC-Z replaced by YFP

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. PIK3CD gRNA (BRDN0001146545)

    Plasmid

    #76165

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3CD

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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