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Lyn11-targeted FRB (LDR)

Plasmid

#20147

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-linker-FRB (LDR) (MTOR Human)

Expression

Mammalian

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pET28b-PunyEEER

Plasmid

#208322

Purpose

Bacterial expression of the His-tagged peptide PunyEEER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PunyERRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-PunyEEER

Plasmid

#208322

Purpose

Bacterial expression of the His-tagged peptide PunyEEER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PunyERRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TinyERRE

Plasmid

#208320

Purpose

Bacterial expression of the His-tagged peptide TinyERRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TinyERRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TinyERRE

Plasmid

#208320

Purpose

Bacterial expression of the His-tagged peptide TinyERRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TinyERRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-TagBFP

Plasmid

#113086

Purpose

General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-TagBFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-TagBFP

Plasmid

#113086

Purpose

General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-TagBFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTOX4

Plasmid

#127449

Purpose

Encodes YhaV toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

yhaV toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX4

Plasmid

#127449

Purpose

Encodes YhaV toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

yhaV toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-kanMX6

Plasmid

#26882

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-kanMX6

Plasmid

#26882

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCBP60g-NLS-3xmCitrine

Plasmid

#192521

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

CALMODULIN BINDING PROTEIN 60g

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

CBP60g

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCBP60g-NLS-3xmCitrine

Plasmid

#192521

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

CALMODULIN BINDING PROTEIN 60g

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

CBP60g

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-NLRP10

Plasmid

#38141

Depositor

Thomas Kufer

Article

Lautz et al Cell Microbiol. 2012 Jun 4. doi: 10.1111

Insert

NLRP10 (NLRP10 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only