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Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

Plasmid

#208038

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-BE4-NG

Plasmid

#200448

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-NS1NF

Plasmid

#175279

Purpose

AAV vector mediating inducible expression of the NS1 gene of YFV-17D

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1 (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

tight TRE

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCBmut

Plasmid

#164605

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp16

Plasmid

#157724

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp16 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp5-HF

Plasmid

#157687

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp5

Plasmid

#157686

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp5 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-Puro-sgAMPKa1

Plasmid

#138684

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF17_P2A_Hygro_Barcode

Plasmid

#120503

Purpose

Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF17 (KLF17 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF2_P2A_Hygro_Barcode

Plasmid

#120489

Purpose

Barcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF2 (KLF2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xControl

Plasmid

#245729

Purpose

Express 42xControl mRNA in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xControl mRNA

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xRCGG/CGA/AGG

Plasmid

#245728

Purpose

Express 42xRCGG/CGA/AGG mRNA in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xRCGG/CGA/AGG

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-E95A-Tev-3xFlag

Plasmid

#245726

Purpose

Express CNOT3-E95A-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-E95A-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

E95A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-Tev-3xFlag

Plasmid

#245724

Purpose

Express CNOT3-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-K105S-Tev-3xFlag

Plasmid

#245727

Purpose

Express CNOT3-K105S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-K105S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

K105S

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-R59S-Tev-3xFlag

Plasmid

#245725

Purpose

Express CNOT3-R59S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-R59S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

R59S

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBetaActin-IC2N-dCer3

Plasmid

#191325

Purpose

Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

IC2N-dCer-FRB (Dync1i2 Mouse)

Expression

Mammalian

Mutation

aa 1-237

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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