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mCerulean3-CD36-C-10

Plasmid

#55405

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

CD36 (CD36 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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MAS1L-Tango

Plasmid

#66426

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

MAS1L (MAS1L Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pGEM-7Zf-TYR

Plasmid

#32786

Depositor

Ruth Halaban

Article

Ujvari et al J Biol Chem. 2001 Feb 23;276(8):5924-31.

Insert

Tyrosinase (TYR Human)

Use

In vitro transcription

Mutation

I181M

Promoter

T7

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_TLOC1 (220aa)

Plasmid

#45273

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
M72-IRES-tauRFP2-ACNF TV

Plasmid

#15512

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M72-IRES-tauRFP2-ACNF TV (Olfr160 Mouse)

Use

Mouse Targeting

Mutation

Targeting vector to insert IRES-tauRFP2-ACNF imme…

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pMyc2ElbLuc,

Plasmid

#53244

Purpose

reporter plasmid containing 2 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc2ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
PCD FL1P

Plasmid

#12567

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

One perfect CXCR4 binding site (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLJC6-Flag CASTOR1 I280A

Plasmid

#84497

Purpose

Lentiviral expression of CASTOR2 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

sestrin2

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 R152Q GFP

Plasmid

#51884

Purpose

expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 R152Q (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

R152Q: near the extracellular side of the fourth …

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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OXGR1 -Tango

Plasmid

#66466

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

OXGR1 (OXGR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
P2RY13-Tango

Plasmid

#66472

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

P2RY13 (P2RY13 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pTNos:BASTA:PNos (GB0023)

Plasmid

#68211

Purpose

Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

bar gene

Use

Unspecified

Mutation

BsaI and BsmBI sites removed

Promoter

PNos

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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PCD FL6X

Plasmid

#12566

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

Four CXCR4 imperfect binding sites (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-HA CASTOR2 ACT2

Plasmid

#84494

Purpose

overexpression of CASTOR2 ACT2 domain (aa 171-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT2 (GATSL2 Human)

Tags

HA

Expression

Mammalian

Mutation

171-last amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR2 ACT1

Plasmid

#84493

Purpose

overexpression of CASTOR2 ACT1 domain (aa 1-171)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT1 (GATSL2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-171 amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA CASTOR1 ACT2

Plasmid

#84492

Purpose

overexpression of CASTOR1 ACT2 domain (aa 169-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT2 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Mutation

169-last amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR1 ACT1

Plasmid

#84491

Purpose

overexpression of CASTOR1 ACT1 domain (aa 1-169)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT1 (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-169 amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge