We narrowed to 15,981 results for: LEA

1. pET21-pelB-LaM-1

   Plasmid

   #172767

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-1
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC61

   Plasmid

   #118877

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-yHS1

   Plasmid

   #159153

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1
   

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pXFokI-dCas9

   Plasmid

   #60901

   Purpose

   Dual Expression Vector for FokI-dCas9 and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-V5-Smpd4

   Plasmid

   #120237

   Purpose

   Expresses V5-tagged Smpd4 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Smpd4 (Smpd4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaG-29

   Plasmid

   #172753

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-29
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-neo.ARRDC1

   Plasmid

   #38321

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   ARRDC1 (ARRDC1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCHOP10.pCDNA1

   Plasmid

   #21899

   Depositor

   David Ron

   Article

   Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

   Insert

   CHOP10 (Ddit3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

9. NG0886 pCI-TPI-WT-4H

   Plasmid

   #65801

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Tags

   4H (4 copies of short beta-globin 3'UTR for …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. JW617

    Plasmid

    #64809

    Purpose

    CUC3 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Tags

    GAL4 DNA binding domain and Myc

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mADAM17deltaMP in pQCXIP cFLAG

    Plasmid

    #19144

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 189-378

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-Nd1

    Plasmid

    #191523

    Purpose

    Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY012 (pcDNA3.1-hCMtCpf1)

    Plasmid

    #69984

    Purpose

    Expresses humanized CMtCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hCMtCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN-X-hPGK-PuroR-Z

    Plasmid

    #218904

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    hPGK-PuroR
    

    Expression

    Mammalian

    Mutation

    246T>A and 398T>C

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. JW624

    Plasmid

    #64808

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Tags

    GAL4 DNA binding domain

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPOR1CB0013

    Plasmid

    #117549

    Purpose

    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hyper7 in pT3TS-Dest

    Plasmid

    #194296

    Purpose

    In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    Hyper7
    

    Use

    In vitro transcription of mrna

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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