We narrowed to 15,824 results for: LEA

1. pCMV-LOx-IRES-EGFP

   Plasmid

   #166877

   Purpose

   Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

   Depositor

   Sergey Kasparov

   Article

   Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

   Insert

   Lactate oxidase
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SuExp-mPLD3

   Plasmid

   #201245

   Purpose

   express full length mouse PLD3 protein

   Depositor

   David Nemazee

   Article

   Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

   Insert

   PLD3 (Pld3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRmHA3-CH2F2-CG9866

   Plasmid

   #52286

   Purpose

   Expresses Drosophila Ataxin-7

   Depositor

   Jerry Workman

   Article

   Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

   Insert

   Ataxin-7 (CG9866 Fly)
   

   Use

   Tags

   2xHA 2xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ-Myr-PTEN

   Plasmid

   #206983

   Purpose

   Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   N-terminal myristoylation site

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD24-rnc-sfGFP

   Plasmid

   #51560

   Purpose

   Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   rnc (rnc E. coli)
   

   Use

   Tags

   GLESTCRHASLAVLADERRFSA and superfolder GFP

   Expression

   Bacterial

   Mutation

   Contains additonal aa at the end of the sfGFP in …

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-mNINJ1-D53A-GFP

   Plasmid

   #208778

   Purpose

   MSCV vector expressing D53A mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Changed aspartic acid 53 to alanine (D53A)

   Promoter

   Available since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdSh.U6.gRNAGFP

   Plasmid

   #58255

   Purpose

   U6 promoter-driven single guide RNA complementary to eGFP

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAGFP cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC292

   Plasmid

   #118881

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   mRuby3, HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52545

   Purpose

   Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attP (full length)
   Neo
   PhiC31o integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET41a iGluSnFR

    Plasmid

    #119828

    Purpose

    Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-21

    Plasmid

    #172749

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-21
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

    Plasmid

    #197968

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    C328S, C331S, L1332V

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC291

    Plasmid

    #118867

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mRuby3

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTSSX-STC

    Plasmid

    #115668

    Purpose

    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

    Depositor

    David Kramer

    Article

    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

    Insert

    small tetraheme cytochrome c
    

    Use

    Tags

    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDARMO_CMVT_N_Strep_BIRC6

    Plasmid

    #197972

    Purpose

    Mammalian cell expression of wt Strep-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    L1332V

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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