We narrowed to 19,001 results for: Tec

1. pUCBB-ptet-eGFP

   Plasmid

   #32554

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   His

   Promoter

   ptet

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b+PCNA1 P186C

   Plasmid

   #66173

   Purpose

   Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   P186C

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC1748

   Plasmid

   #127982

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available Since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFPQ4

   Plasmid

   #83559

   Purpose

   Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL7 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   GAL7

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

   Plasmid

   #192412

   Purpose

   To act as a 1-input state of the split-AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

   Plasmid

   #192413

   Purpose

   To act as a 1-input state of the split-AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAPM-D4-miR30-TRIM5

   Plasmid

   #132932

   Purpose

   Lentivector for TRIM5 knockdown and puromycin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   TRIM5 shRNA
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-ML1N*2(7Q)

   Plasmid

   #67679

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2(7Q)
   

   Use

   Gateway multisite 3' entry clone

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPL-Yh2

   Plasmid

   #61650

   Purpose

   Gateway vector for generating yellow fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced yellow fluorescent protein
   

   Expression

   Plant

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

    Plasmid

    #191151

    Purpose

    Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L72:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

    Plasmid

    #171429

    Purpose

    Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C261
    

    Expression

    Mammalian

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTF3-AK (clone3)

    Plasmid

    #21165

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    POUF5F1 (genomic DNA) (POU5F1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    NeoR, EGFP, and SV40 polyA are between the two ge…

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pABM-D4-miR30-L1221

    Plasmid

    #132933

    Purpose

    Lentivector for luciferase (control) knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCBB-pBAD-eGFP

    Plasmid

    #32553

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Promoter

    pBAD

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pASKt15C+SrtA7woH

    Plasmid

    #65020

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 FLIPE-10u

    Plasmid

    #13542

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207R in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay FLIPE-1m

    Plasmid

    #13548

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB6684

    Plasmid

    #106154

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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