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ROS1-dCas9 (D10)

Plasmid

#239487

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-ROS1-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available Since

Feb. 17, 2026

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9 (M14)

Plasmid

#239482

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-GUS

Plasmid

#234916

Purpose

This all-in-one vector is used to express the GUS gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

GUS

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-UNC13A

Plasmid

#244344

Purpose

5' AAVLINK plasmid for UNC13A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

UNC13A (UNC13A Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstar3B

Plasmid

#241225

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3B in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar3B

Expression

Plant

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstarC

Plasmid

#241271

Purpose

Expresses NADPH/NADP+ biosensor NAPstarC in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstarC

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-Bmal1

Plasmid

#239045

Purpose

Overexpression of Bmal1

Depositor

Qingchun Tong

Article

Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

Insert

mArntl (Bmal1 Mouse)

Use

AAV

Promoter

Bmal1

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.sggfp(C)

Plasmid

#236042

Purpose

The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

Plasmid

#213527

Purpose

Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

D90A and E92A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem wt-wt)

Plasmid

#224757

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)

Depositor

Dan Minor

Article

Bagriantsev et al EMBO J. 2012 Aug 1;31(15):3297-308. doi:

Insert

Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-RFP(pLxIS-PLPLR)

Plasmid

#221284

Purpose

Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

RFP (pLxIS-PLPLR) (Mkrn1 Synthetic)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-Gal4DBD-CapN-SsrA-CapC

Plasmid

#213533

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-DENV_NS3-GS-NS2B

Plasmid

#201940

Purpose

Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DENV NS3-GS-NS2B (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-WNV_NS2B-GS-NS3

Plasmid

#203475

Purpose

Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

WNV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SLEV_NS2B-GS-NS3

Plasmid

#203510

Purpose

Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

poly SLEV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoC

Plasmid

#193809

Purpose

CMV overexpression of ChemoC in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC

Tags

10xHis

Expression

Mammalian

Mutation

mCerulean3:T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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MmCavin4-APEX2-P2A-mKate2

Plasmid

#170524

Purpose

Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-sphys-3XHA

Plasmid

#163690

Purpose

plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

triple HA tag insertion into endogenous mouse synaptophysin gene

Use

AAV

Tags

triple HA tag

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer7

Plasmid

#138579

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17918617_17920400

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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