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YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PEST2D

Plasmid

#62434

Purpose

MXS_chaining vector with PEST2D

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Destabilization tag

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJLI-Sup35?M

Plasmid

#1225

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Use

Yeast integrative plasmid

Mutation

deletion of aa 124-253 of SUP35

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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MXS_MCS3

Plasmid

#62397

Purpose

MXS_chaining vector with MCS3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

MluI-XhoI-HindIII-PstI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1deltaRRM

Plasmid

#53257

Purpose

GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

RRM domain deleted (aa 230-355)

Promoter

GAL1/10

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2-P394L

Plasmid

#51744

Purpose

overexpression of Sir2 in S. cerevisiae (P394L mutant), HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Mutation

P394L

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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p316CUPSup35GFPSG

Plasmid

#1224

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Tags

GFPSG S65T V164A

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBACgus4x/cdc37/LRRK2 1867-2186_C2024S/C2025S

Plasmid

#40366

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

aa1867-2186, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO V5 SSA1

Plasmid

#19516

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1230K

Plasmid

#10990

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1230K (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 3688 G to A in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
ClhN-p1k-Sec7-4V5-APEX2-URA

Plasmid

#207060

Purpose

Expressing Sec7-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Sec7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pSEC7

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Snf7-4V5-APEX2-URA

Plasmid

#207058

Purpose

Expressing Snf7-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pSNF7

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pJP_1node

Plasmid

#230984

Purpose

pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression.

Depositor

Yolanda Schaerli

Article

Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

Insert

mCitrine

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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16_GP_Vps4p_L7C_A43C

Plasmid

#203356

Purpose

Vps4 with stabilized MIT domain and 16aa GlyPro N-terminal extension

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

6x-HIS-TEV and Gly Pro linker (16aa)

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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2xPeptide 8_Vps4p_L7C_A43C

Plasmid

#203358

Purpose

Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

2xPeptide 8 and 6x-HIS-TEV

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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Vps4p_GP_linker

Plasmid

#203361

Purpose

Vps4 with GlyPro linker between MIT domain and Large ATPase Domain

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4

Tags

6x-HIS-TEV

Expression

Bacterial

Mutation

Residues 83-111 are replaced with Gly Pro linker

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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