We narrowed to 23,499 results for: CRISPR

1. pZBTB40-donor

   Plasmid

   #112335

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZBTB40 with GFP

   Depositor

   Kevin White

   Insert

   ZBTB40 homology arms flanking EGFP-IRES-Neo cassette (ZBTB40 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZNF192-donor

   Plasmid

   #112334

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF192 with GFP

   Depositor

   Kevin White

   Insert

   ZNF192 homology arms flanking EGFP-IRES-Neo cassette (ZNF192 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZNF212-donor

   Plasmid

   #112331

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF212 with GFP

   Depositor

   Kevin White

   Insert

   ZNF212 homology arms flanking EGFP-IRES-Neo cassette (ZNF212 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZNF324-donor

   Plasmid

   #112327

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF324 with GFP

   Depositor

   Kevin White

   Insert

   ZNF324 homology arms flanking EGFP-IRES-Neo cassette (ZNF324 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZNF408-donor

   Plasmid

   #112323

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF408 with GFP

   Depositor

   Kevin White

   Insert

   ZNF408 homology arms flanking EGFP-IRES-Neo cassette (ZNF408 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZNF398-donor

   Plasmid

   #112319

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF398 with GFP

   Depositor

   Kevin White

   Insert

   ZNF398 homology arms flanking EGFP-IRES-Neo cassette (ZNF398 Human)
   

   Use

   CRISPR

   Mutation

   Unknown

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZNF70-donor

   Plasmid

   #112318

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF70 with GFP

   Depositor

   Kevin White

   Insert

   ZNF70 homology arms flanking EGFP-IRES-Neo cassette (ZNF70 Human)
   

   Use

   CRISPR

   Mutation

   Unknown

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF266-donor

   Plasmid

   #112306

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF266 with GFP

   Depositor

   Kevin White

   Insert

   ZNF266 homology arms flanking EGFP-IRES-Neo cassette (ZNF266 Human)
   

   Use

   CRISPR

   Mutation

   Unknown

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZSCAN32-donor

   Plasmid

   #112304

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF434 with GFP

   Depositor

   Kevin White

   Insert

   ZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZNF165-donor

    Plasmid

    #112302

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF165 with GFP

    Depositor

    Kevin White

    Insert

    ZNF165 homology arms flanking EGFP-IRES-Neo cassette (ZNF165 Human)
    

    Use

    CRISPR

    Mutation

    Unknown

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-dMSK1

    Plasmid

    #165602

    Purpose

    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, S. Pyogenes, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-ddMSK1

    Plasmid

    #165603

    Purpose

    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, S. Pyogenes, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPPC010.AAV

    Plasmid

    #171175

    Purpose

    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    BBa_J23119 and Sp.pCas9, BBa_J23107

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid

    #46911

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP-KRAB fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, HA, and KRAB domain

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    human Synapsin promoter

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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