We narrowed to 166,096 results for: addgene

1. pGDP1 NDM-1

   Plasmid

   #112883

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   NDM-1 (blaNDM-1 Klebsiella pneumoniae)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTS-HA-GFP11

   Plasmid

   #177725

   Purpose

   Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   MTS-HA-GFP11
   

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. gh123

   Plasmid

   #106790

   Purpose

   expression of gRNA targeting ST8SIA3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ST8SIA3 (ST8SIA3 Human)
   

   Expression

   Mammalian

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. nls-transposase-nls-pA in pT3TS-Dest

   Plasmid

   #140873

   Purpose

   Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   nls-transposase-nls (Tol2)
   

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-EN1201

   Plasmid

   #92144

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
   

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_T2A_mCherry sgAAVS1

   Plasmid

   #192480

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dSaCas9-VPR

   Plasmid

   #205251

   Purpose

   In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSaCas9-VPR
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER20-Src(K295R)

   Plasmid

   #215465

   Purpose

   Dox-inducible lentiviral transfer vector with Src kinase-dead mutant (K295R)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   Src (SRC Chicken)
   

   Use

   Lentiviral

   Mutation

   K295R (Kinase dead)

   Promoter

   mCMV, Doxycycline-inducible

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. XRE-H2B-eGFP

    Plasmid

    #182294

    Purpose

    Fluorescent reporter for AhR activity

    Depositor

    Severine Degrelle, Thierry Fournier

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    Xenobiotic Response Element (CYP1A1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    XRE

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR v2-sgControl

    Plasmid

    #230080

    Purpose

    Crispr knock out control

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    non-specific sequence control
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWPXL-Sox9

    Plasmid

    #36979

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Marathon-RT pentamutant for Split-PE (pEH107)

    Plasmid

    #190110

    Purpose

    Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, N26R, D74R, N116K, N197R

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-spCas9-SB

    Plasmid

    #169919

    Purpose

    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.

    Depositor

    Zhili Rong

    Article

    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

    Insert

    SpCas9-SB100X
    

    Use

    CRISPR

    Promoter

    CAG

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p27-IRES-mCherry

    Plasmid

    #80143

    Purpose

    retroviral expression of p27 and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    p27 (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh; U6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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