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We narrowed to 10,363 results for: crispr cas9 plasmids

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Showing: 5081 - 5100 of 10363 results

  1. pDONOR 6colors

    Plasmid
    #229974
    Purpose
    bigMamAct assembled plasmid for 6colors cellular labelling
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    See Depositor Comments
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229974-plasmid-map-sequence-id-461259

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  2. psLIB U6

    Plasmid
    #229956
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229956-plasmid-map-sequence-id-461573

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  3. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

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  4. psLIB TRE

    Plasmid
    #229955
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229955-plasmid-map-sequence-id-461253

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  6. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  7. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  8. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  9. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82703
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82703-plasmid-map-sequence-id-158053

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  10. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  14. pZBED9.1.1-gDNA

    Plasmid
    #175854
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZBED9-Nickase1
    Use
    CRISPR
    Available Since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    175854-plasmid-map-sequence-id-349653

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  15. pZBED9.1.2-gDNA

    Plasmid
    #175853
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZBED9-Nickase2
    Use
    CRISPR
    Available Since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    175853-plasmid-map-sequence-id-346180

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  16. pCDX4.1.1-gDNA

    Plasmid
    #175852
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    CDX4-Nickase1
    Use
    CRISPR
    Available Since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175852-plasmid-map-sequence-id-346320

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  17. pCDX4.1.2-gDNA

    Plasmid
    #175851
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    CDX4-Nickase2
    Use
    CRISPR
    Available Since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175851-plasmid-map-sequence-id-346318

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  18. ZMAT4.1.1-gDNA

    Plasmid
    #175871
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZMAT4-Nickase1
    Use
    CRISPR
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175871-plasmid-map-sequence-id-346234

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  19. ZMAT4.1.2-gDNA

    Plasmid
    #175872
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZMAT4-Nickase2
    Use
    CRISPR
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175872-plasmid-map-sequence-id-346232

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  20. HEY2.1.1-gDNA

    Plasmid
    #175873
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    HEY2-Nickase1
    Use
    CRISPR
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175873-plasmid-map-sequence-id-346224

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Showing: 5081 - 5100 of 10363 results