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pDONR223-BUB1

Plasmid

#23612

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1 (BUB1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Human SHIP2

Plasmid

#124645

Purpose

Expresses HIS tagged human SHIP2 in COS-7/HEK cells

Depositor

Christophe Erneux

Publication

Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

Insert

INPPL1 (INPPL1 Human)

Tags

His Xpress (N-terminal)

Expression

Mammalian

Mutation

coding sequence starts at amino acid 18 as calcul…

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Nup43

Plasmid

#98276

Purpose

mammalian expression of SNAPf-Nup43

Depositor

Mike Heilemann

Publication

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup43 (NUP43 Human)

Tags

SNAPf-tag

Expression

Mammalian

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-AKT1

Plasmid

#23752

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT1 (AKT1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Publication

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-EXOSC10

Plasmid

#23674

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EXOSC10 (EXOSC10 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pNF02-mJuniper

Plasmid

#219398

Purpose

Constitutive expression of mJuniper in E. coli

Depositor

Christian Lesterlin

Publication

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mJuniper

Expression

Bacterial

Promoter

proDp

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Publication

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Human, Synthetic)

Tags

6x His and mCherry

Expression

Mammalian

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40571

Depositor

Daniel Gerlich

Publication

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor N-terminally fused to histone 2B

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-PinkyCaMP

Plasmid

#232858

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

Depositor

Olivia Masseck

Publication

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP11-His3MX

Plasmid

#91951

Purpose

Universal C-terminal GFP11 tagging

Depositor

Rong Li

Publication

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Type

Empty backbone

Expression

Yeast

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet1

Plasmid

#85742

Purpose

shRNA against mouse Tet1

Depositor

Hongjun Song

Publication

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet1

Use

AAV

Tags

EYFP

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ER_K0

Plasmid

#133863

Purpose

Expression of ER-targeted Ribosome non-stalling reporter

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

ER_K0

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAG31

Plasmid

#35124

Depositor

John McCusker

Publication

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

patMX3

Use

Yeast replicating

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Publication

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PIK4CB

Plasmid

#23839

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK4CB (PI4KB Human)

Use

Gateway Cloning

Mutation

1MM, G799D

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-Spike

Plasmid

#173921

Purpose

Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

Depositor

Andreas Stuermer

Insert

SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)

Tags

no tags, native protein

Expression

Bacterial and Mammalian

Promoter

standard CMV

Available since

April 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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