We narrowed to 11,635 results for: Vars

1. sgRNA BRCA2 E2772K

   Plasmid

   #139323

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 E2772K using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPKm-245

   Plasmid

   #90503

   Purpose

   Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
   

   Use

   Lentiviral

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK669

   Plasmid

   #192638

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dxCas9-NG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dxCas9-NG h…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPKm-244

   Plasmid

   #90502

   Purpose

   Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNR

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
   

   Use

   Lentiviral

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS1983

   Plasmid

   #49114

   Purpose

   AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple253-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   PITX3

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pPKm-112

   Plasmid

   #90494

   Purpose

   pcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   MTAD-PIF3
   

   Expression

   Mammalian

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_DOCK2-1/1

   Plasmid

   #91482

   Purpose

   Protein expression and purification of human SH3 domain construct DOCK2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   DOCK2-1/1 (DOCK2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P CISD2_2

   Plasmid

   #160776

   Purpose

   Suppress CISD2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD2_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1P CISD2_1

   Plasmid

   #160775

   Purpose

   Suppress CISD2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD2_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. gfp-Arkadia/ RNF111 deltaRING

    Plasmid

    #112229

    Purpose

    Expresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cells

    Depositor

    Vasso Episkopou

    Article

    Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

    Insert

    (Arkadia)Rnf111 (Rnf111 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    delta RING domain deletion (Deletion of the last …

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pHH0103_AMPH-1/1

    Plasmid

    #91556

    Purpose

    Protein expression and purification of human SH3 domain construct AMPH-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    AMPH-1/1 (AMPH Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_DBNL-1/1

    Plasmid

    #91409

    Purpose

    Protein expression and purification of human SH3 domain construct DBNL-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DBNL-1/1 (DBNL Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-A0LWB3

    Plasmid

    #103070

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_ARHGEF16-1/1

    Plasmid

    #91334

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF16-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF16-1/1 (ARHGEF16 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_ARHGEF26-1/1

    Plasmid

    #91342

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF26-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF26-1/1 (ARHGEF26 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT526

    Plasmid

    #180513

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe EGFR Del5(T790M)

    Plasmid

    #32069

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Delete E746-S752, insert V, T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

18. HEL binding scFv D44.1

    Plasmid

    #111718

    Purpose

    Yeast Surface Display of D44.1 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1
    

    Tags

    cmyc tag

    Expression

    Yeast

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p1.2-GS

    Plasmid

    #162770

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-K1LM04

    Plasmid

    #103119

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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