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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Expression

Bacterial

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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RAB31

Plasmid

#25552

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RAB31 (RAB31 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 25, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pWZL Neo Myr Flag NUAK2

Plasmid

#20552

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NUAK2 (NUAK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pST1374-D10A-GCN4

Plasmid

#113025

Purpose

Expresses D10A-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

D10A-GCN4 (GCN4 S. pyogenes, Budding Yeast)

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ctrl)

Plasmid

#69215

Purpose

Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
SFFV promoter
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGR475

Plasmid

#213784

Purpose

Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

Depositor

Chang Liu

Insert

WT TP-DNAP1 (recoded)

Use

Synthetic Biology

Expression

Yeast

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHygG2

Plasmid

#200066

Purpose

hygR selection nls-mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-CEP41_Halo

Plasmid

#135446

Purpose

Mamalian cell expression of HaloTag7 localized at the centrosome

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

CEP41-HaloTag7

Tags

CEP41

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYX223-mtGFP

Plasmid

#45051

Depositor

Benedikt Westermann

Article

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtGFP

Expression

Yeast

Promoter

GAL

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only
pYX113-mtGFP

Plasmid

#45049

Depositor

Benedikt Westermann

Article

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtGFP

Expression

Yeast

Promoter

GAL

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pBa.GFP-KIF13A tail 361-1749

Plasmid

#134622

Purpose

Mammalian expression of mouse KIF13A 361-1749

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13A (Kif13a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

Plasmid

#122954

Purpose

Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NLB (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xhis tag

Promoter

CAG promoter

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10T

Plasmid

#65427

Purpose

Parent vector for constructing non-replicating lesion-bearing plasmid

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBT344_pattB-pCA-GFP-pA-FRT5

Plasmid

#52550

Purpose

for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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ph2p

Plasmid

#22520

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Insert

MPyV VP2

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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