Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 20,609 results for: nes

Clear keywords
Showing: 5121 - 5140 of 20609 results

  1. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Publication
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

    Enlarge

  2. pBa.GFP-KIF16B tail 458-1317

    Plasmid
    #134624
    Purpose
    Mammalian expression of human KIF16B 458-1317
    Depositor
    Marvin Bentley
    Publication
    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF16B (KIF16B Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    WT with deletion of the 457 N-terminal residues.
    Promoter
    Chicken Beta actin
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    134624-plasmid-map-sequence-id-267813

    Enlarge

  4. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104912-plasmid-map-sequence-id-206066

    Enlarge

  7. pcDNA3.1-dCas9-CibN

    Plasmid
    #60552
    Purpose
    Expresses dCas9-CibN in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CibN
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60552-plasmid-map-sequence-id-97051

    Enlarge

  9. GFP-DroshaS300/302A

    Plasmid
    #62530
    Purpose
    Serines 300 and 302 of Drosha are mutated to alanines
    Depositor
    Bharat Ramratnam
    Publication
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serines 300 and 302 to alanines
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. KIF2C

    Plasmid
    #25551
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF2C (KIF2C Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  18. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid
    #122090
    Purpose
    U6 driven SaCas9 sgRNA expression vector for cloning own guides
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122090-plasmid-map-sequence-id-242296

    Enlarge

Showing: 5121 - 5140 of 20609 results