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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Publication

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TFAP4(E135A/S139A)

Plasmid

#110157

Purpose

Expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Publication

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154G/H250Y

Plasmid

#108492

Purpose

expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Publication

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-Flag-AuroraB KD (K106R)

Plasmid

#108488

Purpose

expression of Flag-AuroraB kinase death (K106R)

Depositor

Susanne Lens

Publication

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

Flag

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Publication

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hSTARR-seq_SCP1 vector_blocking 3

Plasmid

#99318

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1000-1600

Plasmid

#89101

Purpose

mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1000-1600

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1601-1900

Plasmid

#89102

Purpose

mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1601-1900

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-230-670

Plasmid

#89099

Purpose

mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 230-670

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pReporter_10

Plasmid

#60570

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_10

Expression

Bacterial

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-IV

Plasmid

#53440

Purpose

Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus (with D29Y mutation in agrD)

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

D to Y mutation of residue 29 of the agrD gene (c…

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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PLK3 335-646-pEGFP

Plasmid

#23267

Depositor

Wei Dai

Publication

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 335-646

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pET20b-delta133HtrA2 S142A

Plasmid

#16153

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S142A

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S400D

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pJS2

Plasmid

#253727

Purpose

CRISPRi system for Pseudomonas aeruginosa expressing sgRNA from the rhamnose-inducible rhaBAD promoter

Depositor

Deborah Hung

Publication

Sullivan et al mBio. 2026 Feb 11:e0276725. doi: 10.1128

Type

Empty backbone

Expression

Bacterial

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Venus-MOA

Plasmid

#234741

Purpose

Mammalian expression of mitochondria marker Venus-MOA

Depositor

Nevin Lambert

Publication

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-MOA

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_SeV_RdRP

Plasmid

#228808

Purpose

Expression of Sendai virus RdRP

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

RdRP

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP3667

Plasmid

#222353

Purpose

Expression vector with GFP expressed under PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Publication

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP12049

Plasmid

#222355

Purpose

Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

Depositor

Jason Peters

Publication

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Publication

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM2 (PITPNM2 Human)

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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