Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,065 results for: LEA

Clear keywords
Showing: 5141 - 5160 of 14065 results

  1. HLTF

    Plasmid
    #28152
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    helicase-like transcription factor (HLTF Human)
    Tags
    thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains HIRAN domain (residues 51 to 171).
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  8. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Publication
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

    Enlarge

  17. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Publication
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

    Enlarge

  18. pJWK_VD_02

    Plasmid
    #158103
    Purpose
    5'UTR stem-loop luciferase reporter: -30kcal/mol
    Depositor
    Ted Dawson
    Publication
    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5'UTR synthetic stem-loop -30kcal/mol
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    158103-plasmid-map-sequence-id-308747

    Enlarge

  19. pJWK_VD_01

    Plasmid
    #158102
    Purpose
    5'UTR stem-loop luciferase reporter: 0kcal/mol
    Depositor
    Ted Dawson
    Publication
    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5'UTR synthetic stem-loop 0kcal/mol
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    158102-plasmid-map-sequence-id-308741

    Enlarge

  20. pASPIre3

    Plasmid
    #154844
    Purpose
    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study
    Depositor
    Markus Jeschek
    Publication
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    Expression
    Bacterial
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154844-plasmid-map-sequence-id-307642

    Enlarge

Showing: 5141 - 5160 of 14065 results