We narrowed to 15,981 results for: LEA

1. pLV-EF1a-V5-Vrk2

   Plasmid

   #120240

   Purpose

   Expresses V5-tagged Vrk2 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Vrk2 (Vrk2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xEGFP-mMeikin (E156R, R159E)

   Plasmid

   #174710

   Purpose

   Mammalian expression of mouse Meikin with Separase-resistant mutations

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin (Meikin Mouse)
   

   Tags

   3xEGFP

   Expression

   Mammalian

   Mutation

   E156R, R159E

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC-CRISPR-35S

   Plasmid

   #99861

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-ChRger3-TS-EYFP

   Plasmid

   #127241

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CamKIIa

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAimR

   Plasmid

   #98687

   Purpose

   Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

   Depositor

   Rotem Sorek

   Article

   Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

   Insert

   AimR
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   Native

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIPZ shCUX1-5328

   Plasmid

   #100815

   Purpose

   Lentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099

   Depositor

   Alain Nepveu

   Article

   Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

   Insert

   TGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG-8

   Plasmid

   #172761

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-8
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaG-5

   Plasmid

   #172760

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-5
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7 shRNA against CRTC1

   Plasmid

   #72710

   Purpose

   Lentiviral expression of shRNA against CRTC1 (3rd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   CRTC1
   

   Use

   Lentiviral

   Tags

   CMV-EGFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pResQ shDna2'

    Plasmid

    #31952

    Depositor

    Sheila Stewart

    Article

    Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

    Insert

    shRNA against hDna2 (DNA2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pRSFDuet-1_VNp-DARPinOFF7

    Plasmid

    #182397

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DARPinOFF7
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL11023

    Plasmid

    #117542

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S_OMEGA(pICH51288)

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. IBMc097

    Plasmid

    #161945

    Purpose

    Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
    

    Expression

    Bacterial

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TR- ATG-HP-GFP

    Plasmid

    #80592

    Purpose

    Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Inserted 30 nt hairpin which is targeted by Csy4 …

    Promoter

    CBA

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL2

    Plasmid

    #184130

    Purpose

    For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Pmrp-1

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1

    Plasmid

    #184129

    Purpose

    For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Pmrp-1 (3kb)

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKO.1-shPrc1

    Plasmid

    #160959

    Purpose

    Prc1 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Prc1 shRNA (Prc1 Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PcDNA-DEST47-CypB-Del3 C-term GFP

    Plasmid

    #36136

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 39-216 of cyclophilin B; G63…

    Promoter

    CMV

    Available Since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pROSA26-G19

    Plasmid

    #21711

    Depositor

    Philippe Soriano

    Article

    Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

    Insert

    ROSA26 G19 (Gt(ROSA)26Sor Mouse)
    

    Expression

    Bacterial

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only