We narrowed to 18,038 results for: Mut

1. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20945

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   hSyn

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pV1326

   Plasmid

   #111435

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-CCR5

   Plasmid

   #98943

   Purpose

   Mammalian expression plasmid for untagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. scFLARE2

   Plasmid

   #158700

   Purpose

   Neuronal expression of scFLARE2 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE2
   

   Use

   AAV

   Tags

   HA, Flag, VP16

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   Syn

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. K44A dynamin 1 pEGFP

   Plasmid

   #34681

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FLAG-Rheb

   Plasmid

   #19996

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Ras homolog enriched in brain 1 (RHEB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO-p53-shRNA-427

    Plasmid

    #25636

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-427 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRVL-3

    Plasmid

    #104581

    Purpose

    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L234A/L235E/G237A and E318A/K320A/K322A

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST_EWSR1

    Plasmid

    #26377

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gRNA-ura-HYB

    Plasmid

    #64330

    Purpose

    ura3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of ura3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

20. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge