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pME-QF

Plasmid

#61375

Purpose

Middle Gateway Entry Vector Containing QF

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QF

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

His tag (N-term and C-term)

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPTn2P-PTK

Plasmid

#85604

Purpose

Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Passport transposon with a puromycin-thymidine kinase fusion

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCascade_SPS1(PmcCAST)

Plasmid

#224926

Purpose

Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

Depositor

Leifu Chang

Article

Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

Inserts

Cas5, Cas6, Cas7, Cas8, Cas11
CRISPR assay for crRNA expression

Use

CRISPR

Expression

Bacterial

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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Duet011

Plasmid

#17627

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pJOE_pvdJ

Plasmid

#110142

Purpose

Suicide plasmid with repair template for knockout in P. putida

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Type

Empty backbone

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-CD9-10

Plasmid

#58076

Purpose

Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

CD9 (CD9 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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p3692 HPV-16 E1

Plasmid

#10859

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

HPV-16 E1 (E1 Human papillomavirus)

Expression

Mammalian

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only

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iDuet101a

Plasmid

#17629

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - EF-1a promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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ath5:GFP-CAAX

Plasmid

#105958

Purpose

transgenesis, EGFP membrane

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

CAAX

Tags

GFP

Expression

Bacterial

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-Hyg

Plasmid

#48994

Purpose

Gateway adapted transfer of Hygromycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

hygromycin B phosphotransferase

Use

Gateway attb1 & attb2

Available Since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4.66

Plasmid

#26951

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

beta-tubulin (tbb-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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tdTomato-CD36-C-10

Plasmid

#58077

Purpose

Localization: Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

CD36 (CD36 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pHdCas9

Plasmid

#137080

Purpose

Expression of Himar-dCas9 in bacteria

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Expression

Bacterial

Promoter

pLtetO-1

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHA-N1

Plasmid

#86049

Purpose

A mammalian expression vector with a HA-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)EYFP

Plasmid

#31306

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)EYFP

Use

Cre/Lox

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRC072

Plasmid

#225982

Purpose

J23110_buj_sfGFP_buj_mRFP

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Insert

sfGFP (with buj RBS), mRFP (with buj RBS)

Expression

Bacterial

Promoter

J23110

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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